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Green Exfoliation of Graphene

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Green Exfoliation of Graphene ( green-exfoliation-graphene )

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Pharmaceutics 2023, 15, x FOR PEER REVIEW 12 of 12 of 15 Figure 9. Relative amount of Nrf2 (a), p65 (b) and HO-1 (c) in HaCaT cells after treatment with incre ing doses of either bG or cG for 24 h. * Statistically significant difference from control (p < 0.05). The treatment with both nanomaterials in THP-1 derived macrophages had a diff ent effect compared to other cell lines. Both bG and cG did not activate Nrf2 in any d tested (Figure 10a) but there was a mild dose-dependent activation of HO-1 in bG-treat cells compared to the control (Figure 10c). Moreover, cG induced a significant increase Figure 9. Relative amount of Nrf2 (a), p65 (b) and HO-1 (c) in HaCaT cells after treatment with Fthigeupre695. aRmelaotuivnetawmhouicnht owf Nasrfa2ls(ao),dpo6s5e(-bd)eapndenHdOe-n1t(c(a) binoHutaC1a-fTocldellcshaaftnergtereantmde2n-tfowlidthcihnacrne increasing doses of either bG or cG for 24 h. * Statistically significant difference from control (p < 0.05). ing doses of either bG or cG for 24 h. * Statistically significant difference from control (p < 0.05). for 20 and 50 μg/mL, respectively) (Figure 10b). The treatment with both nanomaterials in THP-1 derived macrophages had a diff ent effect compared to other cell lines. Both bG and cG did not activate Nrf2 in any do tested (Figure 10a) but there was a mild dose-dependent activation of HO-1 in bG-treat cells compared to the control (Figure 10c). Moreover, cG induced a significant increase the p65 amount which was also dose-dependent (about 1-fold change and 2-fold chan for 20 and 50 μg/mL, respectively) (Figure 10b). Figure 10. Relative amount of Nrf2 (a), p65 (b) and HO-1 (c) in THP-1 derived macrophages after Figure 10. Relative amount of Nrf2 (a), p65 (b) and HO-1 (c) in THP-1 derived macrophages af Pharmaceutics 2023, 15, 993 treatment with increasing doses of either bG or cG for 24 h. * Statistically significant difference from treatment with increasing doses of either bG or cG for 24 h. * Statistically significant difference fr control (p < 0.05). control (p < 0.05). Figure 10. Relative amount of Nrf2 (a), p65 (b) and HO-1 (c) in THP-1 derived macrophages aft e o ga e e g o

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