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2 for medical applications. Graphene and its derivatives possess outstanding mechanical, electronic, optical, thermal, and chemical properties [5] Graphene could be used in the medical fields of tissue engineering, drug delivery and gene delivery [6]. 1. Tissue Engineering Tissue engineering is a multidisciplinary field focused on the application of engineering and biological sciences to create a biological substitute that can repair and improve tissue’s function [7]. Graphene-based materials possess mechanical properties such as high elasticity, flexibility, and adaptability to flat or irregular surfaces that are suitable for the structural reinforcement of materials frequently used for tissue engineering [8]. 2. Drug Delivery Nanographene prepared at low cost with specific surface properties enables to interact with various molecules, which allow then to be used in drug delivery. These nanocarriers have primarily been used for cancer treatment to improve effectiveness and reduce side-effects [6]. For instance GO and the anticancer drug hypocrellin A complex can induce the generation of singlet oxygen molecules which can kill the impregnated cells after exposed to light irradiation [9]. 3. Gene Delivery Graphene nanosheets with large sp2 hybridized carbon area, are able to interact not only with drugs, but also with other molecules like the nucleic acids DNA and RNA. Therefore, they can be used for gene delivery or as carriers and protectors of probes involved in identifying miRNAs [6]. Also, graphene is an option to deliver plasmids, especially whenPDF Image | Hydrodynamic cavitation exfoliation layered graphene nano
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