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For applications particularly sensor and medical uses, graphene has to be functionalized. Keeping this aspect in view, we have functionalized graphene with amino acid: L-cysteine. The lateral size found in the present case is larger (up to ~18μm) as compared to previously reported lateral size of graphene. This improved lateral size will enhance the loading capacity of ligand. Therefore, in order to maintain the nearly defect free nature of the as synthesized graphene sheet it was functionalized non-covalently with use of amino acid i.e.; L-cysteine. The FTIR spectra recorded confirmed the amine functionalization of the graphene sheet. The interpretation of the result is as follow: For functionalization 25 mg of graphene sheets were added to 250 ml of double distilled water and then sonicated for one and a half hour for graphene sheets to obtain homogeneous solution. Then, 0.1 M of L-cysteine (double distilled water) was added and sonicated for 30 min followed by 3 h for graphene sheets of constant stirring. The graphene sheets so obtained were thoroughly washed with double distilled water in centrifuge at 10,000 rpm for 10 min and the solution phase was discarded. This washing was repeated 5 times in order to remove any unbounded L-cysteine. Fig. 8. FTIR spectrum of functionalized graphene with amino acid: L-cysteinePDF Image | Synthesis Graphene Electrochemical Exfoliation of Graphite
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