Curcumin with Either Gramicidin or Ouabain

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compounds induce CS in ABC-transporter-expressing drug- resistant cells. The novel aspect of the CS phenomenon reported here is that two compounds, which are individually neither toxic to the resistant nor to the parental cells, can evoke CS in resistant cells when they act synergistically. The work presented here therefore reveals a mechanism of CS that can be targeted rationally because it uses a known phenotypic differ- ence (i.e. inducible ATP depletion by overexpressed MDR transporters) to kill resistant cells. Acknowledgments—We thank Dr. Susan Bates (NCI) for the generous gift of the ABCG2-expressing cell lines. We also thank Dr. Suneet Shukla, and Joseph Koos for guidance in membrane preparation and ATPase assays and Gowthamy Venkidasubramonian for assistance in maintaining cell lines. Finally, we thank Dr. Gary Luker for excel- lent suggestions and a clinical perspective of the relevance of MDR efflux pumps. REFERENCES 1. Szakács, G., Hall, M. D., Gottesman, M. 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