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8 74 7 3 3 I f the sample bemg tested reqUIres more extensIve dl1utlOn than IS provIded by Table 1, an mtermed1ate d11utlOn IS prepared from 20 ml of sample dl1uted to 200 ml wIth odor-free water Use thIs dl1utlOn for the threshold determmation Multiply the T O N obtamed by ten to correct for the mtermed1ate dl1utlOn In rare cases more than one tenfold mtermed1ate dlIutlOn step may be reqUIred Based on the results obtamed m the prehmlnary test, prepare a set of dl1utlOns usmg Table 2 as a gUIde One or more blanks ale mserted m the senes, m the v1c1mty of the expected threshold, but aVOld1ng any repeated pattern The observer does not know whIch dl1utlOns are odorous and wmch are blanks He smells each flask m sequence, begmmng wIth the least concentrated sample and companng wIth a known flask ofodor- free water, untl1 odor IS detected wIth utmost certamty Table 2 DIlutions for Various Odor IntenSIties - Sample Volume m WhICh Odor First Noted 200 ml 50 ml 12 5 ml 3 1 ml Volume (ml) of Sample to be Dlluted to 200 ml 7 5 Record the observatlOns of each tester by mdlCatmg whether odor IS noted (+ SIgn) m each test flask For example ml sample dIluted to 200 ml Response CalculatlOns 125 o 25 o 50 100 200 + +++ 8 1 8 2 The threshold odor number IS the dl1utlOn ratIo at whIch odor IS Just detectable In the example above (7 5), the first detectable odor occurred when 25 ml sample was dl1uted to 200 ml Thus, the threshold IS 200 dIVIded by 25, equals 8 Table 1 hsts the threshold odor numbers that correspond to common dl1utlOns Anomalous responses sometImes occur, a low concentratlOn may be called posItive and a hIgher concentratlOn m the senes may be called negatIve In such a case, the threshold IS deSIgnated as that pomt ofdetectlOn after whlCh no further anomahes occur 200 100 50 25 100 50 50 25 25 125 125 63 (mtermedlate DIlutIOn See 733) 125 63 31 140 1-5PDF Image | Methods for Chemical Analysis of Water and Wastes
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