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7 5 Dl1ute each tube to 50 ml wIth dIstIlled water, add 2 0 ml of Nessler reagent (6 6) and mIX After 20 mInutes read the absorbance at 425 nm agaInst the blank From the values obtaIned plot absorbance vs mg NH3-N for the standard curve DetermIne the ammOnIa In the dIstIllate by nesslenzIng 50 ml or an allquot dl1uted to 50 ml and readIng the absorbance at 425 nm as descnbed above for the standards AmmonIa-nItrogencontentISreadfromthestandardcurve 743 PotentIOmetnc determInatIOn Consult the method entItled NItrogen, AmmOnIa Selective Ion Electrode Method (Method 350 3) In thIS manual It IS not ImperatIve that all standards be dIstl1led In the same manner as the samples It IS recommended that at least two standards (a hIgh and low) be dIstl1led and compared to sIml1arvaluesonthecurvetoInsurethatthedIstIllatIOntechnIqueISrellable IfdIstl1led standards do not agree WIth undistIlled standards the operator should find the cause of the apparent error before proceedIng 8 CalculatIOns . N = A x 02~ x 1,000 N=Axri,000 x ~ 8 1 8 2 TItnmetnc 742 Colonmetnc determInatIOn Prepare a senes of Nessler tube standards as follows ml of Standard 10 ml = 001 mg NH3-N 00 05 10 20 30 40 50 80 100 mg NH3-N/50 0 ml 00 0005 001 002 003 004 005 008 010 where A= mlO02NH2S04used S = mlsample Spectrophotometnc mg/lNH1 - mg/lNH _ 1 where A = mg NH3-N read from standard curve B= mltotaldIstIllatecollected,IncludIngboneaCIdanddIlutIOn C = ml dIstIllate taken for nesslenzatIOn D = ml ofongInal sample taken 3502-4PDF Image | Methods for Chemical Analysis of Water and Wastes
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