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7.2 If necessary, filter the sample through a 0 45 u pore SIze filter USing the first portlOn of filtrate to nnse the filter flask 73 Place 50 ml of sample, or an ahquot dl1uted to 50 ml, in a 50 ml Nessler tube, hold untIl preparatlOnofstandardsIScompleted 74 At the same tIme prepare a senes ofstandards in 50 ml Nessler tubes as follows ml of Standard SolutiOn 10 ml = 0001 mg N02-N 00 05 10 15 20 30 40 50 100 Cone, When Dtluted to 50 ml, mg/l of N02-N (Blank) 001 002 003 004 006 008 010 020 75 Add 2 ml ofbuffer-color reagent (6 2) to each standard and sample, mIX and allow color to develop for at least 15 minutes The color reactlOn medmm should be between pH 1 5 ' and 2 0 7.6 Read the color in the spectrophotometer at 540 nm agamst the blank and plot concentratlOn ofN02-N agamst absorbance 8. CalculatIon 8 1 Read the concentratlOn ofN02-N duectly from the curve 82 Ifless than 50 0 ml ofsample IS taken, calculate mg/l as follows NO N II _ mg/lfromstd curve X 50 2 - ,mg - ml sample used 9 PreCISIon and Accuracy 9 1 PrecIsIon and Accuracy data are not aval1able at thIS time BIblIography 1 Standard Methods for the ExammatlOn for Water and Wastewater, 14th EditlOn, p 434, Method 420, (1975) 3541-2PDF Image | Methods for Chemical Analysis of Water and Wastes
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