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42 4 1 2 Total Hydrolyzable Phosphorus (P-hydro)-phosphorus m the sample as measured by the sulfunc aCId hydrolysIs procedure, and mmus predetermmed orthophosphates ThIs hydrolyzable phosphorus mc1udes polyphosphates [(P20 7)-4, (P3olOyS, etc] plus some orgamc phosphorus (00669) 4 1 3 Total Orgamc Phosphorus (P-org)-phosphorus (morgamc plus oXIdIzable orgamc) m the sample as measured by the persulfate dIgestIOn procedure, and mmus hydrolyzable phosphorus and orthophosphate (00670) DIssolved Phosphorus (P-D) - all of the phosphorus present m the flltrate of a sample filtered through a phosphorus-free fllter of 0 45 mICron pore SIze and measured by the persulfatedIgestIOnprocedure (00666) 421 DIssolved Orthophosphate (P-D, ortho) - as measured by the dIrect colonmetnc analySISprocedure (00671) 422 DIssolved Hydrolyzable Phosphorus (P-D, hydro) - as measured by the sulfurIC aCId hydrolysIs procedure and mmus predetermmed dIssolved orthophosphates (00672) 423 DIssolved Orgamc Phosphorus (P-D, org) - as measured by the persulfate dIgestIOn procedUJ.:e, and mmus dIssolved hydrolyzable phosphorus and orthophosphate (00673) The followmg forms, when sufficIent amounts of phosphorus are present m the sample to warrant such consIderatIOn, may be calculated 431 Insoluble Phosphorus (P-I)=(P)-(P-D) (00667) 4 3 44 5 Interferences 5 1 5 2 5 3 5 4 No mterference IS caused by copper, Iron, or sIhcate at concentratIOns many times greater than theIr reported concentratIOn m sea water However, hIgh Iron concentratIOns can cause preCIpItatIOn of and subsequent loss of phosphorus The salt error for samples rangmg from 5 to 20% salt content was found to be less than 1% Arsenate IS determmed sIml1arly to phosphorus and should be conSIdered when present m concentratIOns hIgher than phosphorus However, at concentratIOns found m sea water, It does not mterfere Sample turbIdIty must be removed by flltration pnor to analySIS for orthophosphate Samples for total or total hydrolyzable phosphorus should be flltered only after dIgestIon Sample color that absorbs m the photometnc range used for analySIS WIll also mterfere 43 1 1 43 12 43 I 3 Insoluble orthophosphate (P-I, ortho)=(P, ortho) - (P-D, ortho) (00674) Insoluble Hydrolyzable Phosphorus (P-I, hydro) = (P, hydro) - (P- D, hydro) (00675) Insoluble Orgamc Phosphorus (P-I, org) = (P, org) - (P-D, org) (00676) All phosphorus forms shall be reported as P, mg/l, to the thIrd place 6 Apparatus 6 1 Techmcon AutoAnalyzer consIstmg of 365 1-3PDF Image | Methods for Chemical Analysis of Water and Wastes
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