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5 6 Apparatus 61 Photometer - A spectrophotometer or filter photometer sUitable for measurements at 650 or 880 nm With a hght path of 1cm or longer 62 ACid-washed glassware All glassware used should be washed With hot 1 1 Hel and rmsed With distilled water The aCid-washed glassware should be filled With dist111ed water and treated With all the reagents to remove the last traces of phosphorus that might be adsorbed on the glassware Preferably, thIS glassware should be used only for the determmatiOn of phosphorus and after use It should be nnsed WIth dlst111ed water and 42 4 1 3 Total Orgamc Phosphorus (P, org) - phosphorus (morgamc plus oXidizable orgamc) m the sample measured by the persulfate digestiOn procedure, and mmus hydrolyzablephosphorusandorthophosphate (00670) Dissolved Phosphorus (P-D) - all of the phosphorus present In the filtrate of a sample filtered through a phosphorus-free filter of 0 45 micron pore Size and measured by the persulfatedigestiOnprocedure (00666) 42 1 Dissolved Orthophosphate (P-D, ortho) - as measured by the direct colonmetnc analysis procedure (00671) 422 Dissolved Hydrolyzable Phosphorus (P-D, hydro) - as measured by the sulfunc aCid hydrolysIs procedure and mmus pre-determmed dissolved orthophosphates (00672) 4 2 3 Dissolved Orgamc Phosphorus (P-D, org) - as measured by the persulfate digestiOn procedure, and mmus dissolved hydrolyzable phosphorus and orthophosphate (00673) The folloWIng forms, when suffiCient amounts of phosphorus are present m the sample to warrant such consideratiOn, may be calculated 43 1 Insoluble Phosphorus (P-I)= (P)-(P-D) (00667) 43 44 Interferences 5 1 5 2 5 3 many times 43 1 1 43 12 43 1 3 Insoluble orthophosphate (P-I, ortho)=(P , ortho)-(P-D, ortho) (00674) Insoluble Hydrolyzable Phosphorus (P-I, hydro)=(P, hydro)-(P-D, hydro) (00675) Insoluble Orgamc Phosphorus (P-I, org)=(P , org) - (P-D, org) (00676) All phosphorus forms shall be reported as P, mg/l, to the thud place No Interference IS caused by copper, Iron, or sihcate at concentratiOns greater than their reported concentratiOn In sea water However, high iron concentratiOns can cause precipitatiOn ofand subsequent loss ofphosphorus The salt error for samples rangIng from 5 to 20% salt content was found to be less than 1% Arsenate is determIned siml1arly to phosphorus and should be considered when present In concentratiOns higher than phosphorus However, at concentrations found In sea water, it does not mterfere 3652-3PDF Image | Methods for Chemical Analysis of Water and Wastes
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