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42 413 Total Orgamc Phosphorus (P, org) - phosphorus (Inorgamc plus oXidizable orgamc) In the sample measured by the persulfate digestiOn procedure, and mInUS hydrolyzablephosphorusandorthophosphate (00670) Dissolved Phosphorus (P-D) - all of the phosphorus present In the filtrate of a sample filtered through a phosphorus-free filter of 0 45 miCron pore Size and measured by the persulfatedigestiOnprocedure (00666) 42 1 Dissolved Orthophosphate (P-D, ortho) - as measured by the direct colonmetnc analysis procedure (00671) 422 Dissolved Hydrolyzable Phosphorus (P-D, hydro) - as measured by the sulfunc aCid hydrolysis procedure and mInUS pre-determIned dissolved orthophosphates (00672) 4 2 3 Dissolved OrganIc Phosphorus (P-D, org) - as measured by the persulfate digestiOn procedure, and mInUS dissolved hydrolyzable phosphorus and orthophosphate (00673) The follOWIng forms, when suffiCient amounts of phosphorus are present In the sample to warrant such conSideratiOn, may be calculated 4.3 1 lnsolublePhosphorus (P-I)= (P)-(P-D) (00667) 4 3 44 5. Interferences 5.1 5 2 Arsenate is determIned siml1anly to phosphorus and should be conSidered when present ThiS Interference may be ehmInated by redUCIng the arsemc aCid to arsemous aCid With SodIUm bisulfite (7 4) When high concentratiOns of iron are present low recovery of phosphorus w111 be obtaIned because it w111 use some of the redUCIng agent The bisulfite treatment w111 also ehIDlnate thiS Interference 6. Apparatus 6.1 6 2 6.3 Photometer-A spectrophotometer or filter photometer SUitable for measurements at 660 or 880 nm With a 11ght path of! cm or longer AC1d-washed glassware All glassware used should be washed With hot 1 1 HCI and nnsed With distl1led water The aCid-washed glassware should be filled With distilled water and treated w1th all the reagents to remove the last traces of phosphorus that IDlght be absorbed on the glassware Preferably, thiS glassware should be used only for the determInatiOn of phosphorus and after use it should be rInsed With distl1led water and kept covered untl1 needed agaIn I f this is done, the treatment With 1 1 HCI and reagents 1SonlyreqUiredoccaSiOnally Commercialdetergentsshouldneverbeused Water bath, 95°C 43 1 1 43 12 43 13 Insoluble orthophosphate (P-I, ortho)= (P, ortho) - (P-D, ortho) (00674) Insoluble Hydrolyzable Phosphorus (P-I, hydro)=(P , hydro) - (P-D, hydro) (00675) Insoluble Orgamc Phosphorus (P-I, org)=(P, org) - (P-D, org) (00676) All phosphorus forms shall be reported as P, mg/l, to the third place 3653-2PDF Image | Methods for Chemical Analysis of Water and Wastes
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