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64 6 5 PreparatIon ofStandards 641 If dIgestIOn (62) was used add 200 mg NaHC03 (52) and 24 ml H2S04 (5 3) to standards to compensate for sIhca Introduced by these reagents and for effect of the salt on the color IntensIty Photometnc measurement 66 VIsual ComparIson 6 7 Correction for color or turbIdIty 6 7 1 A speCIal blank IS run USIng a portion of the sample and carryIng out the procedure m6 1,6 2 Ifused, and 63 except for the addItion ofammomum molybdate:6 J 2) 6 5 1 Prepare a cahbration curve USIng approxImately SIX standards to span the range shown below wIth the selected hght path Se1ection of LIght Path Length for Vanous SIhca ConcentratIons 6 5 2 6 5 3 Carry out the steps In 6 3 USIng dIstilled water as the reference Read a blank Plot photometnc readIng versus ug of sIhca In the final solutIOn of 54 5 ml Run a reagent blank and at least one standard wIth each group ofsamples 6 6 1 SIhca value mg 000 010 020 040 050 075 Prepare a set of permanent artIfiCIal color standards accordIng to the table Use well stoppered, properly labelled 50 ml Nessler tubes LIght Path cm 1 2 5 10 SIhca In 54 5 ml final volume (ug) 200-1300 100-700 40-250 20-130 PotaSSiUm chromate solutIOn (591) ml Borax solutIOn (592) ml DIstIlled water ml 00 25 30 10 25 29 20 25 28 40 25 26 50 25 25 75 25 22 100 100 25 20 662 6 6 3 Venfy permanent standards by companson to color developed by standard sII1ca solutIOns These permanent artifiCIal color standards are only for color companson i>rocedure, not for photometnc procedure 370 1-3PDF Image | Methods for Chemical Analysis of Water and Wastes
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