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1 BIOCHEMICAL OXYGEN DEMAND Method 405.1 (5 Days, 20°C) STORET NO. 00310 Carbonaceous 80082 Scope and AppllcatlOn l i T h e blOchemlcal oxygen demand (BOD) test IS used for determmmg the relative oxygen reqmrementsofmumclpalandmdustnalwastewaters AppllcatlOnofthetesttoorgamc waste discharges allows calculatlOn of the effect of the discharges. on the oxygen resources of the recelvmg water Data from BOD tests are used for the development of engmeenng cntena for the design of wastewater treatment plants 12 The BOD test IS an empmcal bioassay-type procedure which measures the dissolved oxygen consumed by microbial hfe whl1e asslml1atmg and oXldlzmg the orgamc matter present The standard test conditions mc1ude dark mcubatlOn at 20°C for a specified time penod (often 5 days) The actual environmental conditions of temperature, blOloglcal populatlOn, water movement, sunhght, and oxygen concentratlOn cannot be accurately reproduced m the laboratory Results obtamed must take mto account the above factors when relatmg BOD results to stream oxygen demands Summary of Method 2 1 The sample of waste, or an appropnate dl1utlOn, IS mcubated for 5 days at 20°C m the dark The reductlOn m dissolved oxygen concentratlOn dunng the mcubatlOn penod yields a measure of the blOchemlcal oxygen demand Comments 31 DetermmatlOn of dissolved oxygen m the BOD test may be made by use of either the Modified Wmkler with Full-Bottle Techmque or the Probe Method m this manual 32 AddltlOnal mformatlon relatmg to oxygen demandmg charactenstlcs of wastewaters can be gamed by applymg the Total Orgamc Carbon and Chemical Oxygen Demand tests (also found m this manual) 33 The use of 60 ml mcubatlOn bottles m place of the usual 300 m1 mcubatlOn bottles, m conjunction With the probe, IS often convement PrecISion and Accuracy 41 Eighty-SIX analysts m fifty-eight laboratones analyzed natural water samples plus an exact mcrement of blOdegradable orgamc compounds At a mean value of 2 1 and 175 mg/l BOD, the standard devlatlOn was ±O 7 and ±26 mg/l, respectively (EP A Method Research Study 3) 42 ThereISnoacceptableprocedurefordetermmmgtheaccuracyoftheBODtest 2 3 4 Approved for NPDES CBOD pendmg approval for Section 304(h), CWA Issued 1971 Edltonal revIsion 1974 405 1-1PDF Image | Methods for Chemical Analysis of Water and Wastes
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