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823 Waltanother5mInutesandadd7mlofworkIngbuffersolution(76) 8 2 4 After 15 mInutes, read the absorbance at 520 nm agaInst a reagent blank The color IS stable for 4 hours 83 Concentration below 50 ug/1 8 3 1 8 3 2 8 3 3 8 3 4 8 3 5 9 CalculatIon To 500 ml ofdIstIllate In a separately funnel, add 4 ml ofMBTH solutIOn (7 3) After 5 mInutes, add 2 5 ml ofcenc ammoma sulfate solutIOn (7 4) After an addItIOnal 5 mInutes, add 7 ml of workIng buffer solutIOn (7 6) After 15 mInutes, add 25 ml of chloroform Shake the separatory funnel at least 20 tImes Allow the layer to separate, and pass the chloroform layer through filter paper Read the absorbance at 490 nm agaInst a reagent blank 9 1 Prepare a standard curve by plottmg absorbance agaInst concentratIOn values 9 2 ObtaIn concentratIOn value ofsample dIrectly from prepared standard curve 10 PreCIsIon and Accuracy 101 PreCISIOnandaccuracydataarenotavaIlableatthIStime Bibliography 1 Fnestad, H 0 , Ott, E E , and Gunther, F A , "Automated Colonmetnc MICro DetermmatIOn of Phenol by OXIdatIve CouplIng wIth 3-Methyl-2-benzothlazohnone Hydrazone", Techmcon InternatIOnal Congress (1969) 2 Gales, M E , "An EvaluatIOn ofthe 3-Methyl-2 benzothIazolmone Hydrazone Method for the DetermmatlonofPhenolsmWaterandWastewater",Analyst,100,No 1197,841(1975) 4203-3PDF Image | Methods for Chemical Analysis of Water and Wastes
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