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7 Procedure 71 SetupmanifoldasshownInFlg\lre1 8 7 4 7 5' CalculatlOn 8 1 Prepare standard curve by plotting peak heights of processed NTA standards against 6 10 Worlang solutlOn A DIlute 10 0 ml of stock NTA solutlOn to 100 0 ml with distIlled water 10ml=01mgNTA Preparedally 6 11 6 12 6 13 Working solutlOn B DIlute 10 0 ml of SolutlOn A to 100 0 ml with distIlled water 1 0 ml = 001 mgNT A Preparedally Working solutlOn C DIlute 10 0 ml ofSolutlOn B to 100 0 ml with distIlled water 10 ml = 0 001 mg NTA Prepare dally Prepare a senes of standards by dIluting sUItable volumes of worlang solutlOns to 100 0 ml with distilled water ,The following dIlutlOns are suggested 72 73 Allow both colonmeter and recorder to warm up for 30 minutes Run a basehne with all reagents, feeding distilled water through the sample hne Adjust dark current and operative opemng on colonmeter to obtam sUItable baseline PlacewashwatertubesIn samplerIn setsoftwo,leaVingeverythirdposItionvacant Set samphng time at 1 5 minutes ml of SolutIOn C/100 ml 2 4 6 8 10 ml of SolutIon B/100 ml 2 4 6 8 10 ml of Solution A/100 ml Cone, mg NTA/I 002 004 006 008 010 020 040 060 080 100 2 20 4 40 6 60 8 80 10 100 Place NTA standards In sampler Complete filling ofsample tray with unknown samples SWitch sample line from diStilled water to sampler and begin an~lysls known concentratlOns Compute concentratlOn of samples by companng sample peak heIghts wIth standard curve 4302-3PDF Image | Methods for Chemical Analysis of Water and Wastes
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