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3108 Bull. Korean Chem. Soc. 2014, Vol. 35, No. 10 Notes Figure 2. Schemetic flow diagram of SFE modular system. used for supercritical fluid extraction. Carbon dioxide (99.99 wt % pure) was provided by Bakryoung Special Gas (Chun- chon, Korea). All solvents were HPLC grade from Fisher Scientific Korea Ltd. SFE System. Supercritical fluid extractions of curcumin were performed using a JASCO (Tokyo, Japan) LC-900 SFE system. The schematic diagram of the system is shown in Figure 2. This system consisted of three sections: fluid delivery, extraction, and collection. The fluid delivery section includ- ed a high pressure pump, which delivered supercritical fluid CO2. In the extraction section, supercritical fluid extractions were performed with supercritical fluid CO2. The collection section included a back pressure regulator, which kept the pressure of an extraction vessel at the desired value. The effluent flowing through the back pressure regulator reduced its pressure to atmospheric and thereby solutes in the effluent reduced their solubility to virtually zero. In this way, the solutes were deposited and collected in a collection vessel. Since we used pure CO2 as a extracting solvent, the extracts were collected without solvent in the collection vessel. HPLC Analysis. Supercritical fluid extracts of curry powder were analyzed by high performance liquid chromatography (HPLC). The HPLC system consisted of a JASCO PU-980 pump, an UV-2075 UV detector and a Waters Spherisorb S5 Figure 3. HPLC chromatograms of curcumins ((a) typical SFE extract from a curry powder, (b): standard curcumin, (c): standard DMC, (d): standard BDMC).PDF Image | Rapid and Efficient Extraction of Curcumins SCO2
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