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Topics in Current Chemistry

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Topics in Current Chemistry ( topics-current-chemistry )

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Biologically Produced Sulfur 177 Apart from the nature of the sulfur in the globules, the surface of these globules has been studied as well. Studies on sulfur globules produced by Al. vinosum showed that the sulfur particle is bounded by a protein mem- brane, which appears to be sulfur-free [7, 18]. The proteins of the membrane were isolated by extraction and separated by reversed-phase HPLC, reveal- ing three major proteins [37]. Proteins associated with sulfur globules of Thiocapsa roseopersicina appeared to correspond to the proteins on the sulfur globules of Al. vinosum. Later research was able to locate the genes responsible for the production of these proteins and it was concluded that sulfur globules in Al. vinosum are extracytoplasmic due to the presence of this protein cell membrane [27]. Such a cell membrane was also found for sulfur globules in B. alba, where it was called sulfur inclusion envelope [17]. The exact function of the protein is still not clear. Because of the similarity to proteins such as keratin or plant cell wall it has been suggested that the proteins have a structural rather than an enzymatic function [29]. The pro- teins might also prevent the crystallization of the sulfur in the globule [38]. 3.2 Extracellularly Stored Sulfur Sulfur globules produced by bacteria excreting the globules outside the cell membrane have also been studied. Especially for biotechnological applica- tions these sulfur compound oxidizing bacteria are interesting since they al- low an easy separation of the sulfur from the bacteria. In Fig. 5 sulfur glob- ules can be seen which have been excreted by a Thiobacillus bacterium. Steudel proposed a number of models for the composition of bacterial sulfur globules in which he did not always distinguish between intracellular- ly and extracellularly stored sulfur. In a model proposed for sulfur globules excreted by Acidithiobacillus ferrooxidans [39] the globules consist of a sul- fur nucleus (mainly S8 rings and small amounts of other sulfur rings) and Fig. 5 Scanning electron micrograph of sulfur excreting Thiobacillus [40]

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