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Synthetic Biology Capabilities – Advanced genetic toolbox in place • Unique genetic toolbox for gas fermenting organisms developed Robust DNA transfer Scalable electroporation and conjugation methods Ensures efficient strain construction Reduces time and cost Model-guided design Computer aided design tools (BioCAD) Predictive Metabolic model Validated DNA design algorithms (Codon Usage & RBS) Metabolic knowledgebase for identification of new pathways Comprehensive genetic parts library Modular vector system Validated genetic control parts (promoters, library) Antibiotic free markers Automated construction Automated DNA construction using robotics Advanced sequencing capabilities to QC and troubleshoot strains Reduces time and cost Ensures efficient strain construction Liew et al., 2016, Gas Fermentation – A Flexible Platform for Commercial Scale Production of Low Carbon Fuels and Chemicals from Waste and Renewable Feedstocks. Frontiers Microbiol 7: 694. Nagaraju et al., 2016, Genome editing of Clostridium autoethanogenum using CRISPR/Cas9. Biotechnol Biofuels 9: 219. Efficient genome editing Robust transformation method Scarless gene knock-out and/or Integration (Proprietary tools & CRISPR) Ensures efficient strain constructionPDF Image | Novel gas fermentation technology captures CO-rich gases
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