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Abstract Renewable biofuels such as butanol are being researched due to the continued depletion of fossil fuels. Researchers at MIT have recently discovered a B. Megaterium bacteria that survives under high pressure and anaerobic conditions. They are genetically manipulating the bacteria to produce biofuels such as butanol. Butanol is potentially a great alternative for the ethanol dominated biofuel market since it is more energy dense and less flammable than ethanol. At WPI we are operating a supercritical carbon dioxide extraction system compatible with the organism. Throughout the course of our project our team made many improvements to the system set up and procedure for operation. This paper focuses on the extraction results, trends, and models that were generated when operating the high- pressure extraction system. Overall the system showed a slight increase in the extraction rate when the initial butanol concentration was increased from 1 wt.% to 3 wt.% and when the flow rate of scCO2 was increased. On the other hand, no change in the extraction rate was observed when the pressure of the system was varied from 1500 PSI to 2000 PSI. With the experimental data our team concluded that the extraction unit can extract about 80- 85% of butanol in the first 30 minutes. In order to further the future research of this project, experimental and theoretical models were created. The models allowed us to predict extraction rates and overall mass transfer coefficients for runs that were not completed. Therefore, the models reduce the amount of experimental runs that need to be performed. 2PDF Image | Extraction of Bio-Butanol using Supercritical Carbon Dioxide
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