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chiller, it entered the pump as liquid carbon dioxide. The pump was then purged before being turned on and opening the two valves on the inlet stream of the reactor. Once the pressure in the reactor broke 1000 PSI, the valve to the back-pressure regulator was opened and when the reactor reached the operating pressure, the time for the experiment was started. Sample jars were replaced consistently every 10-15 minutes. 3.1.3 Operating the system While the system was running, one team member stayed by the system to record the time, switch out sample jars, monitor the pressure and temperature of the reactor, and make sure the pump was functioning. The sample jars were set up in series, therefore the two jars that were collecting samples had the diffusing heads, at the end of the lines, submerged in the methanol in the sample jars. However, the next sample jars that were attached to the system had the diffusers lifted out of the methanol to prevent methanol from being suctioned back and forth between the sample jars in series. When the set time intervals were reached, the valve after the back pressure regulator was turned to the already attached next set of sample jars and the diffuser heads were submerged in the methanol. The previous sample jars were removed and capped to prevent methanol from evaporating. The next set of sample jars were then attached with the diffusers at the end of the lines out of the methanol samples that were kept on ice. The pressure of the reactor and back-pressure regulator was monitored for consistency. The pressure gauge on the carbon dioxide inlet line was checked to verify the carbon dioxide tank was not empty. The pump had a chamber above it with an inspection port. The chamber had water in it so that it was visible if the pump was running or not. This inspection port was monitored throughout the experiment to make sure that the pump was continuously flowing supercritical carbon dioxide into the reactor. This is a high pressure sight gage used to saturate the CO2 so it does not remove too much water from the reactor 3.1.4 Shut Down Sample Jars After each 10 to 15 minute increment, when the sample jars were collecting the exit stream, the sample jars were removed and prepared for analysis using the gas 31PDF Image | Extraction of Bio-Butanol using Supercritical Carbon Dioxide
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