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Extraction of Bio-Butanol using Supercritical Carbon Dioxide

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Extraction of Bio-Butanol using Supercritical Carbon Dioxide ( extraction-bio-butanol-using-supercritical-carbon-dioxide )

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chromatograph. First the two sample jars in each series were weighed and the weights were recorded. Then the liquid from the two sample jars in each series were combined and mixed together. The mixture was stirred before a small sample was taken and put into a gas chromatography vial (1.5mL). Another 10 mL sample was taken and put into a separate vial for storage. The storage was used in case the data for a particular run had to be retested by the gas chromatograph. The remainder of the liquid was disposed of in the proper waste containers. This process was done for each set of vials throughout all of the reactor runs performed. System Following the 30 minute to an hour long trials, the system was shut down following a standard set of steps. First, the pump was switched off at the same time that the first valve on the inlet line to the reactor was closed. The second valve, right below the reactor on the inlet stream, was also closed and then opened at the end of the depressurizing process. The carbon dioxide tank was closed and the water chiller was shut off. The heating tape between the backpressure regulator and the sample jars were shut off. The heating jacket was kept on until the depressurizing process was completed. The valve before the back-pressure regulator valve was closed and the heating tape of the metal purging line and valve was turned on to the third setting. The metal purging line was inserted into the cap of the 1000 mL Pyrex jar. The purging line from one of the sample jars was then inserted into the other opening in the cap of the 1000 mL Pyrex jar. Then the depressurizing process could begin by adjusting the valve of the metal purging line. If the line became cold or began to frost over then the valve was closed and waited to heat up. After the reactor was below 1000 PSI, the plastic purging line connected to the reactor could also be used to depressurize the system. The two lines were utilized to bring the pressure of the reactor down to atmospheric pressure. After depressurizing, the heating jacket was removed and the second valve on the inlet stream was also closed. The reactor was then disconnected from the inlet stream and then the clamps holding the reactor in place were also removed. The remaining liquid in the reactor was added to the 1000 mL Pyrex jar where the rest of the residue was collected from depressurizing the system. 32

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