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Chapter 4: Results 4.1 Gas Chromatography A graph containing the output data of the gas chromatograph (GC) was plotted in order to validate the proper functioning of the gas chromatograph as well as the accuracy of the standard samples. In Figure 4.1 the retention time versus the signal intensity is plotted. The graph shows that as the concentration of the standard samples increased the intensity of the peaks recorded by the gas chromatograph also increased. This positive linear trend provides proof the chromatograph and the standard samples follow the expected trend. Figure 4.1: Retention time vs GC-FID intensity signal for different wt.% 4.1.1 Calibration Curves Five separate calibration curves for butanol and methanol mixtures were developed. Of the five calibration curves developed, the curve with the highest r2 value was used to analyze data from experimental runs. All five calibration curves can be seen in Figure 4.2. The calibration curve with the highest r2 value (run 2) can be seen in Figure 4.3. 36PDF Image | Extraction of Bio-Butanol using Supercritical Carbon Dioxide
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