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25000000 20000000 15000000 10000000 5000000 y = 6E+06x + 118225 R2 = 0.9942 GC result area 2 Linear (GC result area 2) 0 01234 wt.% Butanol Figure 4.4: Calibration curve for butanol and water standards The extraction samples obtained during experimental runs were measured using the gas chromatograph. A standard sample was run through the gas chromatograph with each set of unknown samples. This allowed us to ensure that the gas chromatograph was functioning properly for each run and would validate the accuracy of our calibration curve over time. There were a few cases when the standard sample did not match up with our methanol/butanol calibration curve; most likely due to variations in the functioning of the gas chromatograph. In these cases, the set of samples were run through the gas chromatograph a second time. When the samples were run a second time, the standard samples always lined up with the calibration curve. For consistency, the gas chromatograph data was used only when the standard sample agreed with our methanol/butanol calibration curve. 4.2 Experimental Results 4.2.1 Mass Balances For each experimental run, the GC results of the samples were used to complete a mass balance around the system. With the initial amount of butanol added to the reactor recorded, the amount removed during each time interval was calculated and the residue leftover in the reactor was analyzed in the GC to determine the amount of butanol that 38 GC AreaPDF Image | Extraction of Bio-Butanol using Supercritical Carbon Dioxide
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