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remained in the reactor at the end of each experiment. The desired percent error was less than 20% for each run. After analyzing the mass balances for the first several runs, the mass balances were producing results with percent errors greater than 20%. The larger margin of error might be due to butanol escaping through the vent line. With a smaller desired percent error, the system was modified to run in series so the extraction line would bubble through a first sample jar of methanol and then a second, smaller sample jar. This would ensure that butanol in the extraction line would be bubbled through two sample jars before the carbon dioxide could be vented out of the system to exhaust. Running the system with this set up decreased the mass balance percent error to be between 5-20% depending on the run. 4.2.2 Raw Data Results on Extraction Rates for Different Parameters After multiple procedural changes were made to how the extraction system operates a final set of butanol extraction data was obtained to show the effects of parameter changes. The three main parameters that were changed in this extraction system were initial butanol concentration in the extraction vessel, the pressure at which extraction occurred, and the mass flow rate of the scCO2 flowing through the system. These parameters were chosen because they were expected to be able to shift the extraction rate of the system in a favorable direction and possibly influence how the extractor will operate in the future when B. Megaterium is added to the system. Changing Initial Butanol Concentration The initial butanol concentration in the extraction vessel was varied from 1-3 wt.% of a 100 to 150 g starting solution. This range was chosen because the organism that will be producing butanol is predicted to only reach these levels of sustainable production. Figures 4.5-4.7 show the raw data of the results for 1-3 wt.% butanol concentration. 39PDF Image | Extraction of Bio-Butanol using Supercritical Carbon Dioxide
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