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Figure 2 - Area-scale analysis comparison of the surface of theT3 modified Nafion membrane and turned surface of mold T3 (measurements pictured above in Fig. 1) Confocal Microscope Objectives The Olympus LEXT3100 confocal microscope has multiple objective lenses (5x, 10x, 20x, 50x and 100x) available for use. Olympus recommends using the higher(50x and 100x) objectives for three dimensional imaging for best results. This reason for this became evident comparing and contrasting images and measurements generated by the microscope. When the lower objective lenses are used to measure surfaces which have significant steep features, which appear as cliffs in the measurement and will be referred to as cliffs, have significantly more large spikes appear near the cliffs using the lower objective lenses than measurements taken using the higher objective lenses. Also low objective lenses can have areas in the image with many small spikes as shown in the 20x objective lens measurement of the T3 mold as portrayed in Fig. 3. This is compared with the 100x measurement, also of the T3 mold, the small spikes that were on the image of the 20x measurement are not seen on the 100x measurement. Area-scale analysis of 19PDF Image | surface roughness of the Nafion membrane in a PEM fuel cell
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