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Biodegradation The biodegradation of organic material in condensing water was measured with the BOD (Biochemical Oxygen Demand) OxiTop® Control technique (Roppola, 2009). This technique is based on small pressure changes in the airspace of the sample container. In the biodegradation reaction the sample biodegrades due to the presence of bacteria that consume oxygen in their respiration reaction. In some way, it resembles the respiration of higher life forms. The reaction produces carbon dioxide, water, new biomass, and ammonia. In the reaction the carbon dioxide forms the same mole amount as oxygen is consumed. The pressure change emerges when the carbon dioxide formed is absorbed into NaOH (sodium hydroxide) pellets. The water that forms enters the liquid phase or absorbs into the NaOH pellet in the upper part of the sample container. Two moles of water is formed in biodegradation reaction when one mole of oxygen is consumed. The biodegradation reaction is represented by the equation: CxHyOzNw , O2 , Bacteria CO2 , H2O , NH3 , Biomass , Energy The BOD OxiTop Control system contains the sample bottles, the NaOH holder in the neck of the bottle, constant stirring with a magnet, and the measuring head. The controller is separate, and it is used to start the measurement and read the values. The equipment provides 360 measuring points for every measurement and it can be monitored in real-time during measurement. The results can be transferred to the computer directly (Prokkola et al., 2012). 95PDF Image | BIOMASS TO ENERGY AND CHEMICALS HighBio2 Project Publication
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