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Figure 3. Schematic Showing the Acidification Experimental Layout Five different types of seawater were evaluated in this test series. Four of these types were synthetic formulations (designated as IO1, IO2, IO3, IO4) created from instant ocean seawater while the other was actual seawater came from our NRL Key West Florida laboratory facility (KW). The synthetic formulations were prepared at least 16 hours prior to usage to allow the buffer salts to reach equilibrium. Below is the concentrations and measured pH of the seawater solutions. The pH of the solutions varied as a result of equilibration with CO2 gas in the atmosphere. These pH changes had no effect on the overall performance of the acidification cell. All pH measurements were conducted with a standardized Fisher combination glass electrode. Carbon dioxide content of solutions were measured by a UIC Coulometric system (UIC Inc, Joliet, IL 60436) [15]. IO 1 - Synthetic seawater was prepared by dissolving 41.1 g L-1 of Instant Ocean Sea Salt in deionized water at a total volume of 100 liters. The pH of the synthetic seawater was 9.1 ± 0.5. The CO2 content was not measured. IO 2 - Synthetic seawater was prepared by dissolving 35 g L-1 of Instant Ocean Sea Salt (in deionized water at a total volume of 140 liters. The pH of the synthetic seawater was 8.4 ± 0.2. The CO2 content was not measured. IO 3 – Synthetic seawater, using Instant Ocean Sea Salt at 35 g L-1, was supplemented with 90 ppm of sodium bicarbonate and diluted with deionized water to a total volume of 140 liters. The pH of the synthetic seawater was 8.2. The CO2 content was measured to be approximately 100 ppm. 8PDF Image | CO2 from Seawater
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