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Emerging Technologies Microbial Activity Objective: Identify and quantify specific microorganisms in wastewater. Description: March 2013 Technology Summary State of Development: Innovative. prepared 2008 Fluorescence In Situ Hybridization (FISH) for Filamentous and Nitrifying Bacteria Bacteria in activated sludge contains DNA as unique genetic material. DNA sequences unique to individual groups of microorganisms can be used to identify specific microorganisms in a sample containing a mixture of many different types of microorganisms. The process of identifying specific microorganisms is part of the full- cycle 16S Ribosomal Ribonucleic Acid (rRNA) approach by using FISH. Fluorescently labeled 16S rRNA probes are hybridized, stained, and observed under an epifluorescent microscope. This document discusses the on-line version, not the field test kit. FISH was developed in the 1990s and routinely is used in medical fields. More recently it has been applied to the wastewater treatment field, as well as at wetlands. Also, it has potential applicability for monitoring efforts such as tracking fecal organisms (“microbial source tracking”). Comparison to Established Technologies: The microbial detection process is able to positively identify specific microorganisms in a mixed culture. Previously, microbiological tests performed in a laboratory were necessary to identify and enumerate bacteria. This process provides real-time feedback, over laboratory tests that take hours or even days for results. Another advantage of using FISH is that it does not have to be performed on cells that are actively dividing, which makes it a more versatile test. Use of FISH is now fairly common. Available Cost Information: Approximate Capital Cost: Unknown. Approximate O&M Costs: Unknown. Vendor Name(s): Department of Civil and Environmental Engineering at the following universities: University of Illinois, Urbana-Champaign University of Cincinnati North Carolina State University Key Words for Internet Search: Installation(s): Littleton/Englewood Wastewater Treatment Plant Englewood, Colorado 80110 (Profile data were collected monthly since July 1996 during this NSF grant period.) Fluorescence In Situ Hybridization, FISH, 16S rRNA, full-cycle 16S rRNA approach, phylogeny Data Sources: Department of Civil and Environmental Engineering, University of Illinois, Urbana-Champaign, University of Cincinnati, and North Carolina State University. Sidney Biesterfeld, Linda Figueroa, Mark Hernandez, and Phil Russell, Colorado School of Mines, Littleton/Englewood Wastewater Treatment Plant, Englewood, Colorado, and University of Colorado, Boulder, 80309: Use of Fluorescent Oligonucleotide Probes to Characterize Vertical Population Distributions of Nitrifying Bacteria in a Full-Scale Nitrifying Trickling Filter, 1998. 5-4 Wastewater Treatment and In-Plant Wet Weather ManagementPDF Image | Emerging Tech for Wastewater Treatment
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