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Emerging Technologies March 2013 Microbial Activity updated 2012 Technology Summary Nicotinamide Adenine Dinucleotide (NADH) Probes Objective: NADH process probes can be used for aeration control to maintain simultaneous nitrification and denitrification conditions. Description: State of Development: Innovative. (Not currently licensed for sale in the United States as of 2012.) Nicotinamide adenine dinucleotide (NAD) is an intermediate compound in biological reactions that functions to transport electrons from a reduced substrate (i.e., chemical oxygen demand) to the biosynthetic pathways. In its reduced form, it exists as NADH or NADPH and is commonly referred to as reducing power. When light at 340 nm strikes NADH, it fluoresces and emits light at 460 nm. NADH probes emitting 340 nm and detecting 460 nm can be used to monitor the level of reducing power by measuring fluorescence, which indicates the concentration of NADH. The measurement is done using immersed probes with no sampling or subsequent analysis. The SymBio process has applied NADH measurement (along with measurement of the dissolved oxygen level) to control aeration as needed to optimize Simultaneous Nitrification and deNitrification (SNdN) in the same basin. When properly controlled the SNdN process provides denitrifying anoxic microenvironments inside the activated sludge floc at the same time as aerobic nitrifying conditions are provided at the floc surface and in the bulk water. Comparison to Established Technologies: Other technologies that monitor the oxidation/reduction level of the bioreactor are dissolved oxygen probes and oxidation reduction potential (ORP) probes. Both are inferior to NADH as measures of biomass reducing power. ORP measurements include the reducing power contributed by reduced substrates whether the biomass is able to act on them. Dissolved oxygen probes provide information on only a single oxidizing compound (oxygen) and provide no information on the state of the reactor environment when it contains no dissolved oxygen as in anoxic or anaerobic conditions. Available Cost Information: Approximate Capital Cost: 2005 cost for SymBio was approximately $100,000 for one sensor with a process control package. The SymBio process and NADH probe technology are no longer licensed for sale in the United States. For possible site-specific licensing, contact former licensee Ovivo Water (Enviroquip). Approximate O&M Costs: No additional costs for O&M are incurred. Vendor Name(s): Ovivo Water (Enviroquip) Formerly licensed to sell SymBio in the United States 2404 Rutland Dr, Suite 200 Austin, TX 78758 Telephone: 512-834-6029 Email: chintan.parikh@ovivowater.com Key Words for Internet Search: Installation(s): More than 40 municipal applications are in the United States including: Bend OR, Big Bear CA, Lake Elsinore CA, New Philadelphia OH, Perris CA, Pflugerville TX, Rochelle IL, Stonington CT SymBio, NADH, Simultaneous nitrification denitrification, SNdN Data Sources: Trivedi, H., and N. Heinen, “Simultaneous Nitrification/Denitrification by Monitoring NADH Fluorescence in Activated Sludge,” WEFTEC 2000. Chintan Parik, Ovivo Water, June 7, 2012. 5-6 Wastewater Treatment and In-Plant Wet Weather ManagementPDF Image | Emerging Tech for Wastewater Treatment
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