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March 2013 Microbial Activity Objective: Identify specific microorganisms in wastewater. Description: Emerging Technologies Technology Summary State of Development: Emerging. prepared 2008 Fluorescence In Situ Hybridization (FISH) for Phosphorus Accumulating Organisms (PAOs) Bacteria in activated sludge contain DNA as unique genetic material. DNA sequences unique to individual groups of microorganisms can be used to identify specific microorganisms in samples that contain a mixture of many different types of microorganisms. The process of identifying specific PAOs is part of the full-cycle 16S rRNA approach using FISH. Fluorescently labeled 16S rRNA probes are hybridized, stained, and observed under an epifluorescent microscope. This document discusses the on-line version, not the field test kit. Comparison to Established Technologies: The FISH for PAOs microbial detection process is able to positively identify specific microorganisms in a mixed culture. Previously, microbiological tests performed in a laboratory were necessary to identify and enumerate bacteria. This process provides real-time feedback, over laboratory tests that take hours or even days for results. Available Cost Information: Approximate Capital Cost: Unknown. Approximate O&M Costs: Unknown. Vendor Name(s): Department of Civil and Environmental Engineering at the following universities: University of Illinois at Urbana-Champaign University of Cincinnati North Carolina State University Key Words for Internet Search: Installation(s): There are no known installations. Fluorescence In Situ Hybridization (FISH), 16S rRNA, full-cycle 16S rRNA approach, phylogeny Data Sources: Amann, R. I., L. Krumholz, and D. A. Stahl, “Fluorescent-Oligonucleotide Probing of Whole Cells for Determinative, Phylogenetic, and Environmental Studies in Microbiology,” Department of Veterinary Pathobiology, University of Illinois, Urbana, IL 61801, Journal of Bacteriology, 172(2), pp. 762–770, February 1990. Amann, Rudolf, “Monitoring the Community Structure of Wastewater Treatment Plants: A Comparison of Old and New Techniques,” Max-Planck Institut für Marine Mikrobiologie, Arbeitsgruppe Molekulare Ökologie, Celsiusstr. 1, D-28359 Bremen, Germany, FEMS Microbiology Ecology, Volume 25, Issue 3, p. 205, March 1998. Daims, Holger, Niels B. Ramsing, Karl-Heinz Schleifer, and Michael Wagner, “Cultivation-Independent, Semiautomatic Determination of Absolute Bacterial Cell Numbers in Environmental Samples by Fluorescence In Situ Hybridization,” Lehrstuhl für Mikrobiologie, Technische Universität München, 85350 Freising, Germany, and Department of Microbial Ecology, Institute of Biological Sciences, University of Aarhus, 8000 Aarhus, Denmark, Applied and Environmental Microbiology, pp. 5,810–5,818, Vol. 67, No. 12, December 2001. Wastewater Treatment and In-Plant Wet Weather Management 5-13PDF Image | Emerging Tech for Wastewater Treatment
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