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Emerging Technologies March 2013 Microbial Activity prepared 2008 Technology Summary Handheld Advanced Nucleic Acid Analyzer (HANAA) Objective: State of Development: Real-time detection of pathogens in water and Emerging. wastewater. Description: HANAA uses the genetic material of microorganisms in wastewater by performing a Polymerase Chain Reaction (PCR) to detect pathogens. PCR is a technique for enzymatically replicating DNA without using a living organism, such as E. coli or yeast. Like amplification using living organisms, this technique allows for a small amount of DNA to be amplified exponentially. The HANAA is a miniature thermal cycler, which can perform PCR in real time. Commercially these products are available as Bio-SeeqTM and RAZOR®, although they are mostly being used for bioterrorism monitoring purposes. Comparison to Established Technologies: HANAA can be compared to a thermal cycler that is used in laboratories performing extensive molecular biology work. HANAA is a portable version of the thermal cycler and therefore, has the benefit of being used in field where monitoring needs to be performed, without extensive sampling and laboratory analysis time. Available Cost Information: Approximate Capital Cost: Unknown. Approximate O&M Costs: Unknown. Vendor Name(s): Smiths Detection Telephone: 1-908-222-9100 Web site: www.smithsdetection.com Idaho Technology Inc. 390 Wakara Way Salt Lake City, UT 84108 Telephone: 801-736-6354 or 800-735-6544 Fax: 801-588-0507 Email: it@idahotech.com Web site: www.idahotech.com Key Words for Internet Search: Installation(s): Information not available about the installations. Bio-SeeqTM, Smiths Detection, Handheld Advanced Nuclei Acid Analyzer, HANNA Data Sources: Higgins, James, “Handheld Advanced Nucleic Acid Analyzer (HANAA) for Waterborne Pathogen Detection,” WERF publication, USDA, 2001. www.smithsdetection.com Telephone conversation with the vendor. 5-14 Wastewater Treatment and In-Plant Wet Weather ManagementPDF Image | Emerging Tech for Wastewater Treatment
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