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Shock Electrodialysis for Water Purification and Electrostatic Correlations

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Shock Electrodialysis for Water Purification and Electrostatic Correlations ( shock-electrodialysis-water-purification-and-electrostatic-c )

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and porous media in our current system. 4.2.2 Disinfection In addition to using shock electrodialysis for removal of ions, another potential ap- plication would be to use this system for disinfection, since the electric fields in the depleted region are relatively high. On average, the electric field in the depleted region is about 10 V/mm, which is 10-100 times lower than what is often used for electroporation of bacteria (about 750 V/mm [100]). However, two factors are signifi- cantly different in our system as compared to usual electroporation. First, the electric field in the depleted region definitely still varies, and there will be regions in which the electric field will be significantly higher than 10 V/mm. Furthermore, the electric field in our system is not pulsed and the bacteria are constantly exposed to them, which makes it worth investigating whether this constant exposure will lead to cell death. However, even if the electric field does not kill the bacteria, the cells should be polarizable and as such they will prefer not to be in the region of high electric field, in which case we would expect the majority of them to end up in the brine stream. Deng et al [21] suggested that this should be possible, and as such we found it worthwhile to do some preliminary experiments in our system. For this particular experiment (done with the help of Zhifei Ge in the Buie Group), we had prepared solutions of E. Coli K12 (optical density = 0.10) in 10 mM NaCl. In order to obtain an isotonic solution (about 300 mOsm), we also added sucrose to a concentration of 280 mM. The solution was flown through the prototype at 76 μL/min subject to a current of 20.4 A/m2. The results of these experiments for two different porous media aare shown in Table 4.1. The measurements that were used were both the initial and final optical density of the solution and also whether there was any bacterial cell growth in the final solutions upon addition of media and incubation. What we observe from these results is that there are almost no bacteria left in our outlet solutions. Hence, neither of the mechanisms mentioned above are dominant. Instead, it appears that all the bacteria are filtered by the porous media. This fact was confirmed by both the observation of increased pressure required to push fluid 104

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