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weaker signals are likely to be due to X-ray emission from the Gelidiella sp. From the EDS spectrums, it is clear that AgNPs reduced by Gelidiella sp have the weight percentage of silver as 30.15%. SEM technique was employed to visualize the size and shape of AgNPs. The formation of AgNPs as well as their morphological dimensions in the SEM study demonstrated that the average size was from 40-50 nm. The shapes of the AgNPs were proved to be spherical (Fig.6.). Similar phenomenon was reported by Chandran et al (22). Cytotoxicity of silver nanoparticles The invitro cytotoxicity of the AgNPs was evaluated Hep-2 cell lines at different concentrations. Our cytotoxicity analysis of the sample shows a direct dose-response relationship; cytotoxicity increased at higher concentrations. The IC50 value was plotted by taking the concentration of AgNPs on X-axis versus percentage of cell viability on Y- axis (Fig.7). The samples demonstrated a considerable cytotoxicity against the Hep-2 cell lines. The result showed that Hep2 cells proliferation were significantly inhibited by AgNPs with an IC50 value of 31.25 μg/ml of the concentration. As shown in Table.No.1, in the lowest tested concentration (3μg/ml), AgNPs were able to inhibit the cell line’s growth by less than 10%. In contrast the presence of 15.62μg/ml of AgNPs significantly inhibited the cell line’s growth (> 60%). Previous study shows that phytochemicals depletes intracellular antioxidants thereby induced cancer cell death (23). Bhimba et al. Int J Pharm Pharm Sci, Vol 4, Suppl 4, 710-715 Fig. 1: Photography of a) Gelidiella sp. extract. b) Formation of AgNPs Fig. 2: UV-VIS absorption spectra of Ag nanoparticle synthesized from Gelidiella sp. at 1mM silver nitrate. 1 00 90 80 70 60 50 40 30 20 10 0 -10 4000 3500 3000 2500 2000 1500 Wavenumbers (cm-1) 1 000 500 Fig. 3: FTIR spectra of capped silver nanoparticles synthesized using aqueous extract of Gelidiella sp. 712 1489.05 426.32 467.02 1033.37 876.20 855.41 %T 3419.78 524.11 713.07 699.89 2524.03 1650.74 2927.19 1787.58PDF Image | anticancer activity of silver nanoparticles extract of Gelidiella
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