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Bhimba et al. Int J Pharm Pharm Sci, Vol 4, Suppl 4, 710-715 Fig. 7: Effect of AgNPs on Hep2 cell viability CONCLUSION In conclusion, the bio-reduction of aqueous silver ions by the aqueous extract of Gelidiella sp. has been demonstrated. This approach towards the synthesis of AgNPs can be easily scaled up economically and eco-friendly. Applications of the synthesized AgNPs in bactericidal, fungicidal and cytotoxic applications, makes this method potentially for invivo method. ACKNOWLEDGEMENT We are grateful to Dr. Rengaswami, Director, CAS Botany, Madras University for identifying the seaweed. The authors thank, Dr. Sugumaran, Department of Animal Biotechnology, Veterinary College, Vepery, Chennai for TEM analysis, Chemistry Lab for XRD analysis. We also thank Mr. Magesh Peter, NIOT, Chennai for helping us in the initial progress of work. REFERENCES 1. Hutchison JE. “Greener nanoscience: a proactive approach to advancing applications and reducing implications of nanotechnology,” ACS Nano 2008; vol. 2, no. 3, pp. 395–402. 2. Mohanpuria P, Rana NK, Yadav SK. Biosynthesis of nanoparticles: technological concepts and future applications. J Nanopart Res 2008; 10, 507. 3. Savithramma N, Rao ML, Devi PS. Evaluation of antibacterial efficacy of biologically synthesized silver nanoparticles using stem barks of Boswellia ovalifoliolata Bal. and henry and Shorea tumbuggaia. Roxb. J. Bil. Sci. 2011; (In Press). 4. Morones JR, Elechiguerra JL, Camacho A, Holt K, Kouri JB, Ramirez JT, Yacaman MJ. The bactericidal effect of silver nanoparticles. Nanotechnology 2005; 16:2346-2353. 5. Bellantone M, Coleman NJ, Hench LL. Bacteriostatic action of a novel four-component bioactive glass. J. Biomed. Mater Res 2000; 51:484-490. 6. Kanchana A, Balakrishna M. Anti-cancer effect of saponins isolated from solanum trilobatum leaf extract and induction of apoptosis in human larynx cancer cell lines. International journal of pharmacy and pharmaceutical sciences 2011; 3(4):356-364. 7. Unno Y, Shino Y, Kondo F, Igarashi N, Wang G, Shimura R, Yamaguchi T, Asano T, Saisho H, Sekiya S, Shirasawa H. Oncolytic viral therapy for cervical and ovarian cancer cells by sindbis virus AR339 strain. Clin. Cancer Res. 2005; 11(12):4553-4560. 8. Abraham SA, McKenzie C, Masin D, Harasym TO, Mayer LD, Bally MB. In-vitro and in-vivo characterization of doxorubicin and vincristine coencapsulated within liposomes through use of transition metal ion complexation and pH gradient loading. J. Clin. Cancer Res. 2004; 10:728-738. 9. Byrd JC, Lucas DM, Mone AP, Kitner JB, Drabick JJ, Grever MR. Anovel therapeutic agent with in-vitro activity against human B-cell chronic lymphocytic leukemia cells mediates cytotoxicity via the intrinsic pathway of apoptosis. J. Hematol. 2000; 101(11): 4547-4550. 10. Xu H, Yao L, Sung H, Wu L, Chemical composition and antitumor activity of different polysaccharides from the roots Actinidia eriantha, Carbohydr. Pol. 2009; Vol.78, 316-322. 11. Hsin, YH, Chen CF, Huang S, Shih TS, Lai PS, Chueh PJ. The Apoptotic Effect of Nanosilver is mediated by a ROS- and JNK- Dependent Mechanism Involving the Mitochondrial Pathway in NIH3T3 Cells. Toxicol. Lett. 2008; 179:130–139. 12. Park S, Lee YK, Jung M, Kim KH, Chung N, Ahn EK, Lim Y, Lee KH. Cellular Toxicity of Various Inhalable Metal Nanoparticles on Human Alveolar Epithelial Cells. Inhal. Toxicol. 2007; 19:59- 65. 13. Creighton JA, Eadont DG. Ultraviolet-visible absorption spectra of the colloidal metallic elements. J. Chem Soc., Faraday Trans. 1991; 87:3881-3891. Fig. 8: Photography of a) Cytotoxic changes observed Vs b) Control 714PDF Image | anticancer activity of silver nanoparticles extract of Gelidiella
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