Antioxidant Activity Determination of Silver Nanoparticles

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Antioxidant Activity Determination of Silver Nanoparticles ( antioxidant-activity-determination-silver-nanoparticles )

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Molecules 2020, 25, 3191 5 of 24 Scheme 1. Reaction of DPPH radical with hydrogen donor [46]. The ABTS (2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid) method or the TEAC (trolox equivalent antioxidant capacity) is based on generating a cation radical ABTS•+ formed by emitting one electron from the nitrogen atom (Scheme 2). ABTS is usually first oxidized by potassium persulphate [49], manganese dioxide [50], or AAPH (2,2′-azobis-(2-amidino-propane) dihydrochloride) [51] which gives rise to the cation radical ABTS•+. It absorbs at 414, 645, 734, and 815 nm and gives blue-green color. The preferred wavelength is 734 nm because the interference with other absorbing components is minimized. The ABTS•+ reacts with antioxidant leading to the decolorization of a solution in the range of 1–30 min [12,39,41,42]. Scheme 2. Generating of ABTS•+ and its reaction with an antioxidant [52]. The use of ABTS•+ radical has the advantage over the DPPH radical as it can be used in both aqueous and organic media [48]. ABTS method is also useful in studying the effect of pH on the antioxidant activity of various compounds [53]. However, the ABTS assay has some reservations in the overall applications, such as specificity for reaction of different antioxidants, storage of the radical, or processing method conditions [53]. DMPD radical scavenging assay is based on the conversion of transparent N,N-dimethyl-p-phenylenediamine dihydrochloride (DMPD) into the colored radical DMPD•+ in the presence of Fe ions or reactive species, such as hydroxyl radicals (Scheme 3). Antioxidants capable of hydrogen atom transfer decolorize the solution, absorbance decrease at 505 nm [12]. The main disadvantage of using this assay is that DMPD radical is soluble only in water, so there are limitations in using it for hydrophobic antioxidants determination [54].

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