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Molecules 2020, 25, 3191 13 of 24 Figure 2. DPPH scavenging activity of (a) AgNPs prepared by Chinese plants extract [123], copyright permission by Elsevier; (b) AgNPs prepared by walnut green husk extract increasing in concentration and time [124], copyright permission by Dovepress. The DPPH and ABTS assays were also compared by measurements of antioxidant capacity of AgNPs prepared by extract of Allium ampeloprasum L. [137]. The antioxidant capacity was determined at different concentrations (100, 200, 300, 400 and 500 mM). The DPPH and ABTS radical scavenging abilities were dose-dependent, which means that increasing scavenging activities against both radicals with the increasing concentration of AgNPs were observed (for DPPH and ABTS the antioxidant activity was in the range 62.2–82.4% and 64.5–96.8%, respectively). These results can be considered slightly inadequate, because the authors did not compare the antioxidant capacity with that of standard, so it can be only hypothesized that silver nanoparticles prepared by using the extract of Allium ampeloprasum L. exhibit good antioxidant activity [137]. In addition to plant extract, bacterial strains are also used for AgNPs biosynthesis. As an example, the study on the use of Trichoderma atroviride for this purpose can be mentioned [153]. The presented work showed that AgNPs exhibited quite higher DPPH scavenging activity in a concentration-dependent manner with IC50 of 45.6 μg/mL [153]. Biomolecules isolated from microorganisms, such as exopolysaccharides were also used for biosynthesis of AgNPs [154,155]. Promising antioxidant properties were reported by Sivasankar et al. [155], who studied antioxidant capacity of silver nanoparticles prepared by exopolysaccharides isolated from Streptomyces violaceus. They focused on DPPH, NO, and H2O2 scavenging activities, total antioxidant activity, and ferric reducing power assay using ferricyanide (Figure 3). The AgNPs showed the DPPH radical scavenging effect of 89.5% atPDF Image | Antioxidant Activity Determination of Silver Nanoparticles
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