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The morphology of the silver nanoparticles has been analysed using TEM. Figure 3 (a–d) show the images of the particles synthesised under 3, 5, 7 and 10 min of cold plasma exposure. The shape is near spherical and the average diameters of the nanoparticles were 17.9 ± 8.9, 14.3 ± 1.6, 10.6 ± 2.3, and 5.4 ± 1.4 nm, respectively. The High‐resolution transmission electron microscopy (HRTEM) Naimnomagatesriaolsf 2t0h2e0,s1i0lv, 8e7r4nanoparticles synthesised under different plasma exposure times are shown i5nof 10 the Figure 3 (e–g). FiFgiugurere3.3.TTrarannssmiissiionElectronMiicrrossccooppyy(T(TEEMM))imimagaegsesofotfhtehenanaonpoaprtaicrlteicslessynstyhnetshiseesdisuednduenrder didffifefreernenttpplalassmaaeexposuretimes::((aa))33minin,,(b(b))55mmini,n(,c)(c7)m7imn,in(d,)(d1)01m0inm;insi;zseizdeisdtriisbturitbiountiofntohefthe nanoparticles determined from TEM : (e) 3 min, (f) 5 min, (g) 7 min, (h) 10 min; HRTEM of nanoparticles determined from TEM: (e) 3 min, (f) 5 min, (g) 7 min, (h) 10 min; HRTEM of nanoparticles nanoparticles (i) 3 min, (j) 5 min, (k) 7 min, (l) 10 min. (i) 3 min, (j) 5 min, (k) 7 min, (l) 10 min. The fringe spacing is measured to be 0.235, 0.14 and 0.202 nm, which corresponds to the (111), The fringe spacing is measured to be 0.235, 0.14 and 0.202 nm, which corresponds to the (111), (220) and (200) planes of silver. The d spacings are 0.236, 0.220, 0.141 and 0.121, indicating the face‐ (220) and (200) planes of silver. The d spacings are 0.236, 0.220, 0.141 and 0.121, indicating the centred cubic (fcc) crystal structure. The results are consistent with the previous reports in literature face-centred cubic (fcc) crystal structure. The results are consistent with the previous reports in [5,21,22]. The sizes obtained on these samples match with the data obtained in the above DLS literature [5,21,22]. The sizes obtained on these samples match with the data obtained in the above measurements. DLS measurements. 3.2. Antibacterial Properties 3.2. Antibacterial Properties Equal volumes of nanoparticle suspensions were infused onto the filter paper disks that were Equal volumes of nanoparticle suspensions were infused onto the filter paper disks that were added to bacterial plates and the growth inhibition zones were observed after incubation for 24 h. added to bacterial plates and the growth inhibition zones were observed after incubation for 24 h. The variation in the size of the inhibition zones was shown to depend on the plasma exposure The variation in the size of the inhibition zones was shown to depend on the plasma exposure (treatment) (treatment) time, as shown in Figure 4. The diameter of the growth inhibition zone was observed to time, as shown in Figure 4. The diameter of the growth inhibition zone was observed to increase when increase when the plasma exposure (treatment) time was increased. S. aureus was seen to be more the plasma exposure (treatment) time was increased. S. aureus was seen to be more sensitive to the sensitive to the silver nano particles compared to E. coli. Based on the UV‐Vis data, there was a slight silver nano particles compared to E. coli. Based on the UV-Vis data, there was a slight difference in the difference in the silver nanoparticle concentrations in the 5‐min, 7‐min and 10‐min plasma‐treated silver nanoparticle concentrations in the 5-min, 7-min and 10-min plasma-treated samples. However, this antibacterial study showed an inverse relationship between the silver nanoparticle size and the antibacterial activity (Figure 4). These observations align with previous studies where decreased particle size increased the antibacterial effect of silver nanoparticles [23–26].PDF Image | Bactericidal Silver Nanoparticles by Plasma Processing
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