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Biofilm Eradication Using Biogenic Silver Nanoparticles

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Biofilm Eradication Using Biogenic Silver Nanoparticles ( biofilm-eradication-using-biogenic-silver-nanoparticles )

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Figure 1. Color change obtained for silver nanoparticles synthesized (a) control, (b) PchNPs and (c) UV-Vis absorption spectra, after 24 h of reaction. After a 24 h period an 87% yield was obtained from the synthesis. The reaction mixture was centrifuged and the pellet containing the silver nanoparticles was washed with and resuspended in Molecules 2020, 25, 2023 water. The UV-vis spectra after centrifugation showed an absorption band at 442 nm, corresponding 4 of 14 to the SPR band of silver nanoparticles (Figure 2). Figure 2. UV-visible absorption spectrum before and after centrifugation showing the final SPR band Molecules 2020, 25, x FOR PEER REVIEW 5 of 15 of the resuspended PchNPs at 442 nm, corresponding to the stable silver nanoparticles. Figure 3. Effect of different reaction conditions on the synthesis of PchNPs (measured by absorbance Figure 3. Effect of different reaction conditions on the synthesis of PchNPs (measured by absorbance at 440 nm) over several days, where error bars indicate the standard deviation: (a) concentration of at 440 nm) over several days, where error bars indicate the standard deviation: (a) concentration of AgNO3, (b) reaction temperatures, (c) incubation times of the fungal mycelium with water. AgNO3, (b) reaction temperatures, (c) incubation times of the fungal mycelium with water. 2.3. Characterization of PchNPs 2.3.1. ζ-potential: Surface Charge of PchNPs ζ-potential determination showed that PchNPs had a high net negative surface charge, close to −30 mV (−24.8 mV), indicating their colloidal stability in aqueous media.

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