PDF Publication Title:
Text from PDF Page: 005
Molecules 2020, 25, 2023 5 of 14 2.3. Characterization of PchNPs 2.3.1. ζ-potential: Surface Charge of PchNPs ζ-potential determination showed that PchNPs had a high net negative surface charge, close to −30 mV (−24.8 mV), indicating their colloidal stability in aqueous media. 2.3.2. DLS and SAXS: Diameter of PchNPs Molecules 2020, 25, x FOR PEER REVIEW 6 of 15 According to the Small Angle X-Ray Scattering (SAXS) measurement, silver nanoparticle size was According to the Small Angle X-Ray Scattering (SAXS) measurement, silver nanoparticle size 26(2) nm (Figure 4a). DLS characterization showed a single population with a hydrodynamic diameter was 26(2) nm (Figure 4a). DLS characterization showed a single population with a hydrodynamic of ca. 45 nm (Figure 4b), larger than the diameter obtained using SAXS, which is to be expected, since diameter of ca. 45 nm (Figure 4b), larger than the diameter obtained using SAXS, which is to be DLS measures the hydrodynamic diameter of the nanoparticles. expected, since DLS measures the hydrodynamic diameter of the nanoparticles. Figure 4. (a) SAXS curve showing one characteristic correlated distance and (b) DLS measurement Figure 4. (a) SAXS curve showing one characteristic correlated distance and (b) DLS measurement indicating nanoparticles of ca. 45 nm diameter in solution with low polydispersity. indicating nanoparticles of ca. 45 nm diameter in solution with low polydispersity. 2.3.3. Confocal Raman Microscopy: Surface Functional Groups of PchNPs 2.3.3. Confocal Raman Microscopy: Surface Functional Groups of PchNPs The use of fungi for silver nanoparticle production provides improved production capacity due The use of fungi for silver nanoparticle production provides improved production capacity due to the high quantities of secreted substances; these bioactive substances play a dual functional role to the high quantities of secreted substances; these bioactive substances play a dual functional role as as reducing and stabilizing agents to the colloidal nanoparticles [21]. Confocal Raman Microscopy reducing and stabilizing agents to the colloidal nanoparticles [21]. Confocal Raman Microscopy was was used to characterize the surface functional groups capping of the silver nanoparticles (Figure 5). used to characterize the surface functional groups capping of the silver nanoparticles (Figure 5). In In particular, the nature of silver nanoparticles gives rise to Surface-Enhanced Raman Scattering (SERS) particular, the nature of silver nanoparticles gives rise to Surface-Enhanced Raman Scattering (SERS) phenomena, which produce a local field effect at the surface of the nanoparticle. Due to this amplifier phenomena, which produce a local field effect at the surface of the nanoparticle. Due to this amplifier effect, it is possible to enhance the Raman signal of those molecular fragments near the surface of the effect, it is possible to enhance the Raman signal of those molecular fragments near the surface of the nanoparticles, which is of great importance to assess the nature of the stabilizing agent (capping) [22]. nanoparticles, which is of great importance to −a1ssess the nature of the stabilizing agent (capping) [22]. The presence of the band positioned at 230 cm can be attributed to the Ag-N stretching mode, which The presence of the band positioned at 230 cm−1 can be attributed to the Ag-N stretching mode, which may be originated from the fungal extract, providing the nitrogen atoms that will finally take part may be originated from the fungal extract, providing the nitrogen atoms that will finally take part of of the capping of the silver nanoparticle. Even more, bands positioned at the fingerprint region can the capping of the silver nanoparticle. Even more, bands positioned at the fingerprint region can be be attributed to the presence of l-valine and l-alanine, which indicate that proteins from the fungal attributed to the presence of L-valine and L-alanine, which indicate that proteins from the fungal extract are part of the capping, or are near the nanoparticle metallic surface [22]. extract are part of the capping, or are near the nanoparticle metallic surface [22]. 2.4. Colloidal Stability Assays The colloidal stability of PchNPs over pH 3–9 and under conditions of high ionic strength ([NaCl] = 10–500 mM) were evaluated (Figure 6a,b respectively). Aggregation of the nanoparticles generates a decrease in intensity at the Surface Plasmon Resonance (SPR) band, as well as a shift in the visible spectrum towards the infrared. Although PchNPs were stable for most of the evaluated conditions, a decrease at the band corresponding to the SPR band, as well as a slight broadening of the peak, were observed at 100 and 500 mM NaCl and pH 3. These results could be attributed to the neutralization of surface charges of PchNPs, which presented negative net charge at pH 6, resulting in their aggregation. Figure 5. Raman spectrum for PchNPs with superimposed Raman spectra of -valine and -alanine.PDF Image | Biofilm Eradication Using Biogenic Silver Nanoparticles
PDF Search Title:
Biofilm Eradication Using Biogenic Silver NanoparticlesOriginal File Name Searched:
molecules-25-02023-v2.pdfDIY PDF Search: Google It | Yahoo | Bing
Turbine and System Plans CAD CAM: Special for this month, any plans are $10,000 for complete Cad/Cam blueprints. License is for one build. Try before you buy a production license. More Info
Waste Heat Power Technology: Organic Rankine Cycle uses waste heat to make electricity, shaft horsepower and cooling. More Info
All Turbine and System Products: Infinity Turbine ORD systems, turbine generator sets, build plans and more to use your waste heat from 30C to 100C. More Info
CO2 Phase Change Demonstrator: CO2 goes supercritical at 30 C. This is a experimental platform which you can use to demonstrate phase change with low heat. Includes integration area for small CO2 turbine, static generator, and more. This can also be used for a GTL Gas to Liquids experimental platform. More Info
Introducing the Infinity Turbine Products Infinity Turbine develops and builds systems for making power from waste heat. It also is working on innovative strategies for storing, making, and deploying energy. More Info
Need Strategy? Use our Consulting and analyst services Infinity Turbine LLC is pleased to announce its consulting and analyst services. We have worked in the renewable energy industry as a researcher, developing sales and markets, along with may inventions and innovations. More Info
Made in USA with Global Energy Millennial Web Engine These pages were made with the Global Energy Web PDF Engine using Filemaker (Claris) software.
Infinity Turbine Developing Spinning Disc Reactor SDR or Spinning Disc Reactors reduce processing time for liquid production of Silver Nanoparticles.
CONTACT TEL: 608-238-6001 Email: greg@infinityturbine.com (Standard Web Page)