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Nanomaterials 2019, 9, x FOR PEER REVIEW 8 of 20 experiments were performed with the nanoparticles obtained using the optimum conditions along Nanomaterials 2019, 9, x FOR PEER REVIEW 8 of 20 with 0.5 g/L pigment. Naenxopmeartiemriaelsn2t0s1w9,e9,re10p42erformedwiththenanoparticlesobtainedusingtheoptimumconditionsalon8gof20 with 0.5 g/L pigment. 1.2 1.2 0.8 0.8 0.4 0.4 0 0300 400 300 400 0h 2h 0h 4h 2h 6h 4h 12h Absorbance Absorbance 500 W50a0velength6(0n0m) Wavelength (nm) Figure 3. Time course study for generation of AgNPs at 6 mM AgNO3 and 0.5 g/L pigment at pH 12. Figure 3. Time course study for generation of AgNPs at 6 mM AgNO and 0.5 g/L pigment at pH 12. Samples were diluted 60 times. 3 Figure 3. Time course study for generation of AgNPs at 6 mM AgNO3 and 0.5 g/L pigment at pH 12. Samples were diluted 60 times. Samples were diluted 60 times. 3.3. Characterization of Produced Nanoparticles 3.3. Characterization of Produced Nanoparticles 3.3. Characterization of Produced Nanoparticles 3.3.1. Size Estimation by TEM and DLS 3.3.1. Size Estimation by TEM and DLS 3.3.1. Size Estimation by TEM and DLS TEM analysis illustrated a distribution of spherical, hexagonal, rod-shaped and TEM analysis illustrated a distribution of spherical, hexagonal, rod-shaped and triangular-shaped TEM analysis illustrated a distribution of spherical, hexagonal, rod-shaped and triangular-shaped particles ranging from 4 to 41 nm (Figure 4a,b). particles ranging from 4 to 41 nm (Figure 4a,b). triangular-shaped particles ranging from 4 to 41 nm (Figure 4a,b). 6h 24h 12h 36h 24h 36h 600 700 800 700 800 (a) (b) (a) (b) Figure 4. (a,b) TEM micrographs of the synthesized AgNPs. Figure 4. (a,b) TEM micrographs of the synthesized AgNPs. Figure 4. (a,b) TEM micrographs of the synthesized AgNPs. Hydrodynamic size estimation of hypothetical spheres by DLS using number distribution with respect to size shows the existence of nanoparticles from 4 to 60 nm. DLS data also indicates the presence of a higher number of particles within 10 nm range and a lesser number of larger particles (Figure 5), which resembles the TEM distribution quite closely. The polydispersity index value (PDI) of suspension was found to be 0.547, which is well below the acceptable range of 0.7. PDI values arePDF Image | Biosynthesis of Silver Nanoparticles Talaromyces purpurogenus
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