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other impurities that can be observed in the background. The colour scale represents the relative content of elements present in the targeted sample area. EPMA mapping detected the presence of AgNPs in areas where the sample is present. This is evident in the corresponding scanning micrograph image (SL in Figure 7). The semi-quantitative data and the comparison of the sample with background data for the targeted area are provided as supplementary material (Figure S1 and Nanomaterials 2019, 9, 1042 11 of 20 Table S1). Figure 7. EPMA mapping showing semi-quantitative elemental composition of the targeted area. Figure 7. EPMA mapping showing semi-quantitative elemental composition of the targeted area. The scale represents the intensity of the elements present in the mapped area. (Scale bar represents The scale represents the intensity of the elements present in the mapped area. (Scale bar represents 2000 nm). 2000 nm). 3.3.4. Zeta Potential 3.3.4. Zeta Potential The zeta potential of the purified nanoparticles was determined to be −24.8 ± 7.2 mV with a The zeta potential of the purified nanoparticles was determined to be −24.8 ± 7.2 mV with a conductivity of 0.0119 mS/cm, indicating a moderately stable suspension (Figure 8). The biogenic conductivity of 0.0119 mS/cm, indicating a moderately stable suspension (Figure 8). The biogenic AgNPs carry an anionic charge on their surface, which could play an important role in their biological AgNPs carry an anionic charge on their surface, which could play an important role in their actibviotylo. gSicianlcaecmtivoitsyt.cSeilnlucelamr omstemcebllrualanresmaermebnraengeastiavrelynecghaatrivgeldy,cthaergseudr,fathce scuhrafragce cmharygeaffmeacyt the intearfafcetcitotnheoifnntearnacotpioanrtoicflneasnwopitahrtitchlesmweitmhbthraenmese.mPbroasniteisv.ePloysicthivaerlgyecdhanragnedopnarntoicplaerstiecxlehsibexithihbiigther cytohtiogxhiecritcyyftortotxhiecistaymfoerrtehaessoanm[e37r]e.aIsnona[s3t7a]b.lIencaoslltoaibdlealcsoyllsotiedmal,styhsetesmur,ftahceseuffrefactcseaerfefemctsoraeredmomorienant dominant than the bulk effects; therefore, stability depends on factors such as electrostatic than the bulk effects; therefore, stability depends on factors such as electrostatic interactions, surface interactions, surface charges on the particle, properties of the solvent, and van der Waals forces. The charges on the particle, properties of the solvent, and van der Waals forces. The zeta potential of the zeta potential of the suspension indicates stability by electrostatic repulsion, as it measures the suspension indicates stability by electrostatic repulsion, as it measures the effective charge present on effective charge present on the nanoparticle surface. The value of zeta potential was estimated by the nanoparticle surface. The value of zeta potential was estimated by measuring the electrophoretic measuring the electrophoretic mobility. For suspensions stabilized only by electrostatic repulsion, mobility. For suspensions stabilized only by electrostatic repulsion, suspensions with a zeta potential suspensions with a zeta potential of ±30 mV are generally treated as highly stable, whereas for of ±30 mV are generally treated as highly stable, whereas for suspensions stabilized by steric repulsion, suspensions stabilized by steric repulsion, a zeta potential value of ±20 mV is usually accepted as a zeta potential value of ±20 mV is usually accepted as stable [19,38]. stable [19,38].PDF Image | Biosynthesis of Silver Nanoparticles Talaromyces purpurogenus
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