Biosynthesis of Silver Nanoparticles Talaromyces purpurogenus

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Biosynthesis of Silver Nanoparticles Talaromyces purpurogenus ( biosynthesis-silver-nanoparticles-talaromyces-purpurogenus )

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Nanomaterials 2019, 9, x FOR PEER REVIEW 14 of 20 in the sample, giving the final log10(CFU) value as 1. Although AgNPs exhibited slower bactericidal kinetics compared with streptomycin, they both eventually resulted in cell death. As such, in future, it might be prudent to use AgNPs in conjunction with standard antibiotics, so that the efficiency of Nantroamdatietriioanlsa2l01a9n,t9i,b1i0o4t2icscanbemergedwiththepenetrationabilityofAgNPsalongwiththeiro1w4nof20 antibacterial ability. 12 10 8 6 4 2 0 A 32 S2 Control 0 10 20 30 40 50 Time (h) Figure 10. Cell death kinetics for S. epidermidis. Error bars represent standard deviation (n = 3). Figure 10. Cell death kinetics for S. epidermidis. Error bars represent standard deviation (n = 3). A 32 A 32 refers to the MBC dosage for AgNPs (32 μg/mL) and S2 refers to the MBC dosage of streptomycin refers to the MBC dosage for AgNPs (32 μg/mL) and S2 refers to the MBC dosage of streptomycin (2 (2 μg/mL). Control refers to cells grown without AgNPs/Streptomycin. μg/mL). Control refers to cells grown without AgNPs/Streptomycin. 3.6. Anti-Cancer Activity of Biogenic AgNPs 3.6. Anti-Cancer Activity of Biogenic AgNPs Recently, researchers have shown that due to their unique properties, AgNPs possess a therapeutic Recently, researchers have shown that due to their unique properties, AgNPs possess a potential that can be utilized in cancer treatment. Figure 11a shows that, with an increase in nanoparticle therapeutic potential that can be utilized in cancer treatment. Figure 11a shows that, with an increase concentration, the cell survival decreased for all the cell lines, indicating the cytotoxic effect of AgNPs in nanoparticle concentration, the cell survival decreased for all the cell lines, indicating the cytotoxic on cell population. In addition, the data shows that compared to HeLa and HepG2, HEK-293 was more effect of AgNPs on cell population. In addition, the data shows that compared to HeLa and HepG2, resistant to these effects than HeLa and HepG2, since more than 60% of HEK-293 survived exposure to HEK-293 was more resistant to these effects than HeLa and HepG2, since more than 60% of HEK-293 AgNPs concentration of 100 μg/mL. This indicates that AgNPs are somewhat selective towards cancer survived exposure to AgNPs concentration of 100 μg/mL. This indicates that AgNPs are somewhat cell lines. The nanoparticles affected HepG2 most strongly. Therefore, the HepG2 cell line was selected selective towards cancer cell lines. The nanoparticles affected HepG2 most strongly. Therefore, the for studying the anti-cancer activity of AgNPs further in detail. The pigment at 500 μg/mL exhibited HepG2 cell line was selected for studying the anti-cancer activity of AgNPs further in detail. The no antiproliferative activity against the HEK-293 and HeLa cell line (Figure S2 (I,III)), whereas a weak pigment at 500 μg/mL exhibited no antiproliferative activity against the HEK-293 and HeLa cell line activity was detected against the HepG2 cell line, where cell survival was almost 80% after exposure to (Figure S2 (I,III)), whereas a weak activity was detected against the HepG2 cell line, where cell pigment (Figure S2 (II)). No discernible change was observed in the cell morphology after exposure to survival was almost 80% after exposure to pigment (Figure S2 (II)). No discernible change was pigment in any cell line. The presence of bioactive ligands responsible for pigment’s weak cytotoxic observed in the cell morphology after exposure to pigment in any cell line. The presence of bioactive activity on the surface of nanoparticles could explain the strong activity of biogenic AgNPs against the ligands responsible for pigment’s weak cytotoxic activity on the surface of nanoparticles could HepxGpl2aicneltlhleinster.ong activity of biogenic AgNPs against the HepG2 cell line. Log10 (CFU/mL)

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