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B. Adebayo-Tayo et al. Heliyon 5 (2019) e02502 Fig. 7. EDX micrograph of the biosynthesized SNPs. Fig. 8. Antibacterial activity of the biosynthesized SNPs. Table 1 The assessment of biofilm formation by test pathogens. Test pathogen S. aureus ATCC 29213 E. coli ATCC 35218 P. aeruginosa ATCC27853 Citrobacter sp. S. typhi ATCC 14028 E. coli ATCC 11775 Bacillus cereus Biofilm Intensity þþþþ þþþ þþ þþþ þ þ - Fig. 9. The antibacterial activity of OsSNPs against E. coli ATCC 35218. feeding behaviour which enables them to feed on all silver nanoparticles that are less than 50 μm in size. They also reported that the aggregation amount of silver nanoparticles in the gut of the Artemia is dependent on the concentration amount of nanoparticles and the amount of nano- particles consumed by the Artemia. Silver nanoparticles biosynthesized in this work are less than 50 microns as confirmed by the SEM result thus suggesting the aggregation of the biosynthesized SNPs in the gut of the Key: þ 1⁄4 slightly formed, þþ 1⁄4 partially formed, þþþ 1⁄4 formed, þþþþ 1⁄4 strongly formed, - 1⁄4 not formed. Artemia thereby leading to the mortality rate obtained in this work. Methanol extract of Oscillatoria sp. contain some bioactive phyto- chemicals with some unique functional groups which act as capping and stabilizing agent for AgNO3 bio-reduction for silver nanoparticles biosynthesis. 4. Conclusion This present research has been able to biosynthesize silver nano- particles in an eco-friendly manner using methanol extract of Oscillatoria sp. The nanoparticles were thermostable to an extent, spherical shape of 10 nm size and exhibited a strong antibacterial and antibiofilm activity 6PDF Image | Green synthesis of silver nanoparticle Oscillatoria extract
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