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Nanomaterials 2021, 11, x FOR PEER REVIEW 16 of 20 Nanomaterials 2021, 11, 96 16 of 19 A. Effectiveness of silver-loaded PVA Films against E. coli 1.00E+10 1.00E+08 1.00E+06 1.00E+04 1.00E+02 1.00E+00 0 20 40 60 Time (Hours) C. Effectiveness of silver-loaded PVA Films against P. aeruginosa 1.00E+10 1.00E+08 1.00E+06 1.00E+04 1.00E+02 1.00E+00 0 20 40 60 Time (Hours) B. Effectiveness of silver-loaded PVA Films against S. aureus 1.00E+10 1.00E+08 1.00E+06 1.00E+04 1.00E+02 1.00E+00 27mg PVA Powder 270mg 10% PVA Gel Half of 1% film heated @140C for 90min Half of 3% film heated @140C for 90min Half of 5% film heated @140C for 90min 0 20 40 60 Time (Hours) Figure 11. Evaluation of in vitro antimicrobial activity of PVA-silver films against (A) E. coli; (B) S. Aureus and (C) P. Aeruginosa. Figure 11. Evaluation of in vitro antimicrobial activity of PVA-silver films against (A). E.coli.; (B) S. Aureus and (C) P. Aeruginosa. 4.6. Antimicrobial Activity of Aqueous Release Media from Silver—Loaded PVA Films Films with different concentrations of impregnated silver nitrate were incubated 4.6. Antimicrobial Activity of Aqueous Release Media from Silver—Loaded PVA Films in water for up to 10 days, with the supernatant, which contained any released silver, Films with different concentrations of impregnated silver nitrate were incubated in collected and replaced with fresh water every 24 h, with the exception of days 8–10, which water for up to 10 days, with the supernatant, which contained any released silver, col- were sampled as one solution without replacing water in between. When the collected lected and replaced with fresh water every 24 h, with the exception of days 8–10, which supernatants were tested for antimicrobial activity, supernatants collected from 1%, 3%, were sampled as one solution without replacing water in between. When the collected and 5% w/w silver nitrate films only showed differences in bacterial inhibition when supernatants were tested for antimicrobial activity, supernatants collected from 1%, 3%, collected up to 3-days post-incubation for P. aeruginosa (Figure 12A), and up to only 1- and 5% w/w silver nitrate films only showed differences in bacterial inhibition when col- day post-incubation for S. aureus (Figure 12B). Once past those time points, the collected lected up to 3-days post-incubation for P. aeruginosa (Figure 12A), and up to only 1-day supernatants showed similar antimicrobial activity regardless of the silver concentration post-incubation for S. aureus (Figure 12B). Once past those time points, the collected su- in the film. However, all of the collected supernatants showed significant antimicrobial pernatants showed similar antimicrobial activity regardless of the silver concentration in activity against P. aeruginosa when compared to controls, which grew to approximately the film. However, all of the collected supernatants showed significant antimicrobial ac- 3.73 × 107 CFU/mL. Supernatants collected at later dates were less potent against S. aureus tivity against P. aeruginosa when compared to controls, which grew to approximately when compared to the controls, which had approximately 7.00 × 107 CFU/mL of bacteria. 3.73E + 07 CFU/mL. Supernatants collected at later dates were less potent against S. aureus For Figure 12A,B, data from the controls were not included for better comparison between when compared to the controls, which had approximately 7.00E + 07 CFU/mL of bacteria. supernatants collected from the various samples. For Figure 12A,B, data from the controls were not included for better comparison between supernatants collected from the various samples. CFU/mL CFU/mL CFU/mLPDF Image | Hydrogel Forming Dressings Containing Silver Nanoparticles
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