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Nanomaterials 2021, 11, 96 antibiotic loaded PLGA nanofibers we developed a uniform animal orthopedic model for in vivo testing [31]. However, it is very difficult to create a uniform infected burn wound model. We felt it unnecessary to develop an anti-infective animal model because similar materials are already used in humans and controlled bacterial testing in vitro with both gram negative and positive bacteria establish that release profiles allow strong anti1b7aocft1e9- rial effects in the presence of huge loads of bacteria. Nanomaterials 2021, 11, x FOR PEER REVIEW 18 of 20 (A) (B) Figure 12. (A) Colony-forming units (CFU) count results from antimicrobial tests (P. aeruginosa) using supernatants col- Figure 12. (A) Colony-forming units (CFU) count results from antimicrobial tests (P. aeruginosa) using supernatants collected lected from incubation of PVA/silver films over a 10-day period; (B) CFU count results from antimicrobial tests (S. aureus) from incubation of PVA/silver films over a 10-day period; (B) CFU count results from antimicrobial tests (S. aureus) using using supernatants collected from incubation of PVA/silver films over a 10-day period. supernatants collected from incubation of PVA/silver films over a 10-day period. 5. Conclusions Whilst these studies used bacteria growing in planktonic conditions it is possible thatTinhewuosuenodfsialvnedrbausransa,nletifmtfiocroebxitaelnadedditipverinodcosmofmmerocriealtlhyanvailfaebwledwaoyus,ntdhdatretshse- ings is now well established in western countries and, amongst these dressings, there are products such as ActicoatTM 7 and ActicoatTM Moisture Control, which contain nanocrys- talline silver and rely on silver nanoparticles for their anti-infective activity. As discussed in this paper, we have discovered that by subjecting PVA films containing silver nitrate to a sequential oven-drying and heat curing treatment, it is possible to generate cross-PDF Image | Hydrogel Forming Dressings Containing Silver Nanoparticles
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