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Sensors 2020, 20, 6890 18 of 23 Nanoparticles Silica encapsulated gold nanospheres Polydopamine encapsulated gold nanoparticles Gold-silver alloy nanoboxes Gold nanoparticles Gold nanoparticles Gold nanoparticles Silver nanoparticles Gold nanoislands Gold nanoholes Upconverting nanoparticles Upconverting nanoparticles Upconverting nanocrystals encoded magnetic microspheres Upconverting nanoparticles Upconverting nanoparticles RENPs Gold nanoparticles Silver nanoclusters Method SERS-based cell imaging SERS-based imaging SERS SERS-based imaging SERS SERS PEF-based immunoassay PEF-based immunoassay PEF-based fluorescence microscopy Spectroscopy Confocal luminescence imaging Fluorescence microscopy Flow cytometry Microarray immunoassay UCL intensity and lifetime imaging Lifetime imaging Flow cytometry Spectroscopy Table 1. Cont. Number of Analytes 3 3 3 3 2 2 2 3 4 2 6 Shown 2 Potentially 6 7 3 3 3 2 Detection Range - - - - 0.5–100 ng/mL - 0.0556–13.5 nM 0.625–20 nM Detection Limit Reference 0.1–100 - - - - - - - ng/mL - [73] - [74] 6.17 pg/mL 0.68 pg/mL [75] 69.86 pg/mL - [76] 0.41 ng/mL 0.35 ng/mL [96] - [97] 6.2 pM [80] 156 pM 0.05 ng/mL [96] 0.15 ng/mL 0.19 ng/mL - [84] - [88] - [89] 0.01 ng/mL [90] - [91] - [92] - [93] 0.4 nM 0.1 nM [98] 0.2 nM 2.4 nM [99] 5.6 nM 0.2–20 nM 10–100 nM Author Contributions: Writing—original draft preparation, G.J.; writing—review and editing, V.K.; visualisation, G.J. and V.K.; supervision, R.R. and A.M.; funding acquisition, A.M. All authors have read and agreed to the published version of the manuscript. Funding: This research was funded by the European Regional Development Fund through the Research Council of Lithuania according to the Programme Attracting Foreign Researchers for Research Implementation, Grant No. 01.2.2-LMT-K-718–02-0022. Conflicts of Interest: The authors declare no conflict of interest. References 1. Song, Y.; Huang, Y.-Y.; Liu, X.; Zhang, X.; Ferrari, M.; Qin, L. Point-of-care technologies for molecular diagnostics using a drop of blood. Trends Biotechnol. 2014, 32, 132–139. [CrossRef] [PubMed] 2. Nimse, S.B.; Sonawane, M.D.; Song, K.-S.; Kim, T. Biomarker detection technologies and future directions. Analyst 2016, 141, 740–755. [CrossRef] [PubMed] 3. Kosaka, N.; Kogure, A.; Yamamoto, T.; Urabe, F.; Usuba, W.; Prieto-Vila, M.; Ochiya, T. Exploiting the message from cancer: The diagnostic value of extracellular vesicles for clinical applications. Exp. Mol. Med. 2019, 51, 1–9. [CrossRef] [PubMed] 4. Fan, Y.; Wang, S.; Zhang, F. Optical Multiplexed Bioassays for Improved Biomedical Diagnostics. Angew. Chem. Int. Ed. 2019, 58, 13208–13219. [CrossRef]PDF Image | Multiplexed Nanobiosensors: Current Trends in Early Diagnostics
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