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www.nature.com/scientificreports/ Run Factor 1 A: fraction conc. (mg/mL) Factor 2 B: silver nitrate conc. (mMol) Factor 3 C: temperature (oC) Response 1 Particle size (Nm) Response 2 PDI 1 2.75 5 2 0.5 3 3 2.75 1 4 5 3 5 5 3 6 2.75 1 7 5 5 8 0.5 3 9 2.75 5 10 0.5 5 11 2.75 3 12 0.5 1 13 5 1 14 2.75 3 80 31.4 80 25.2 60 28.1 80 45.1 60 55.8 80 33.3 70 54.5 60 28.3 60 50.8 70 31.6 70 40.8 70 24.1 70 38.9 70 38.3 0.269 0.231 0.234 0.175 0.395 0.18 0.204 0.224 0.269 0.247 0.168 0.256 0.202 0.164 Table 2. Design matrix showing trial runs performed for optimization of green synthesis of silver nanoparticles by the aid of pomegranate leaves extract (ethyl acetate fraction) using Box–Behnken Design. Scientific RepoRtS | Vol:.(1234567890) Figure 2. 3D response surface plots for particle size responses at 60 °C (a), 70 °C (b) and 80 °C (c) and PDI responses at 60 °C (d), 70 °C (e) and 80 °C (f). The optimum conditions of synthesis of AgNPs using the ethyl acetate fraction was suggested by numerical optimization where desirability function value closer to 1. The target for each response variable was minimization to the least possible value. The optimum conditions were found to be fraction concentration of 0.83 mg/mL, silver nitrate concentration of 1.15 mMol and at 67.5 °C, with predicted values; particle size (24.1 nm) and PDI (0.202). Characterization of the prepared AgNPs at the optimum conditions. UV–Visible spectrophotom- etry. In order to monitor the formation of AgNPs, their SPR bands were recorded at different time intervals (30, 60, 90, 120 and 150 min) during the green synthesis process. In all measurements, AgNPs exhibit strong SPR band within average of λ max 425 nm. The intensity of the absorbance increases with time, however, the lowest λmax was shown after incubation for 60 min (438 nm), indicating nanoparticles with smaller diameter22. (2020) 10:14851 | https://doi.org/10.1038/s41598-020-71847-5 4PDF Image | pomegranate leaves and their role in green silver nanoparticles
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