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Silver Nano Polyphenol Inclusion Compounds Composites

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Silver Nano Polyphenol Inclusion Compounds Composites ( silver-nano-polyphenol-inclusion-compounds-composites )

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Antibiotics 2018, 7, 76 4 of 12 the composites in the DES medium was close to 90% for all polyphenols. Thus, the product efficacies were clearly higher than those of the composites based on the hydroalcoholic solution of COS. Upon comparison with the treatments without phenolic inclusion compounds, it could be observed that the AgNPs-only treatment attained a lower inhibition than the composites for the COS medium, with mycelium growth even at the highest dose. On the other hand, the DES-based AgNPs-only treatment performed noticeably better than its COS counterpart, albeit it did not completely inhibit growth at a concentration of 250 mg·mL−1 (whereas all the composites did). Therefore, an enhanced fungal growth control activity of the ternary mixtures was evidenced. Figure 3. Radial growth values of P. cinnamomi in the presence of the composites, which consisted of different polyphenol inclusion compounds and silver nanoparticles at different concentrations, either in a chitosan hydroalcoholic solution (COS) or in a deep eutectic solvent (DES). A control (no treatment) and two treatments with AgNPs (in COS and DES) without the inclusion compounds are shown for comparison purposes. Error bars represent the standard deviation across three replicates. The results from the sensitivity tests for P. cinnamomi may also be expressed with the help of EC50 and EC90 indicators (Table 1). The sensitivity of the isolate mainly varied as a function of the preparation media, but also according to the phenolic compound used. In line with the discussion presented above, P. cinnamomi (MYC43) was found to be remarkably more sensitive to the treatments prepared in DES, with EC50 values ranging from 0.1 to 8.9 μg·mL−1 and EC90 values between 77.9 and 184.3 μg·mL−1. For comparison purposes, for the COS in hydroalcoholic solution medium treatments the EC50 values ranged from 171.6 to 279.9 μg·mL−1 and those of EC90 from 450.4 to 963.7 μg·mL−1. Table 1. Effective concentrations that inhibited mycelial growth by 50% and 90% (EC50 and EC90, respectively). Treatment COS AgNPs COS Gallic acid COS Silymarin COS Ferulic acid COS Curcumin DES AgNPs DES Gallic acid DES Silymarin DES Ferulic acid DES Curcumin EC50 (μg·mL−1) 458.4 261.3 261.8 171.6 279.9 13.3 0.1 0.6 0.6 8.9 EC90 (μg·mL−1) 1192.8 455.6 963.7 450.4 487.4 253.3 77.9 107.8 107.8 184.3

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