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Processes 2020, 8, 388 5 of 14 2.8.3. Measurement of Cytomorphological Changes in HCC712 After the adequate growth of HCC712 cells in tissue culture flask, it was treated with different concentrations of Ns-AgNPs and then incubated for 24 h at 37 ◦C in 5% CO2 atmosphere. After the incubation, the gross morphological changes in the cells were examined by bright field microscopy. 2.9. Statistical Analysis All antibacterial and cytotoxic activities were performed with minimum of three replicates. Means, standard deviations, error bars were calculated using Microsoft excel. The error bars are given for each Processes 2019, 7, x FOR PEER REVIEW 5 of 14 bar showing a 95% confidence interval. 184 All antibacterial and cytotoxic activities were performed with minimum of three replicates. 2.10. Ethics Statement 185 Means, standard deviations, error bars were calculated using Microsoft excel. The error bars are given 186 for each bar showing a 95% confidence interval. As this experiment was totally based on in-vitro study using bacterial isolates to evaluate the 187 anti-microbial and anti-biofilm activity of black seed silver nanoparticle, ethical review is not required. 188 2.10. Ethics Statement 3. Results and Discussion 189 As this experiment was totally based on in-vitro study using bacterial isolates to evaluate the 190 anti-microbial and anti-biofilm activity of black seed silver nanoparticle, ethical review is not The synthesis of Ns-AgNPs was primarily authenticated visually and then by using UV–visible 191 required. spectroscopy which has been commonly applied to characterize the synthesized metal nanoparticles. 192 3. Results and Discussion After mixing the silver nitrate into the homogenized extract, a change in colour from pale yellow to tan1-9b3rownishTchoelsoyunrthwesaisoofbNsse-ArvgeNdPsdwuaestporiamsaurirlyfacuethpelnatiscmateodnvriesusaolnlyaancdethpehnebnyoumsienngoUnV,–wvihsiibcleh confirmed 194 spectroscopy which has been commonly applied to characterize the synthesized metal nanoparticles. the formation of AgNPs in the reaction mixture. 195 After mixing the silver nitrate into the homogenized extract, a change in colour from pale yellow to 196 tan-brownish colour was observed due to a surface plasmon resonance phenomenon, which 3.1. UV-Vis Spectrum 197 confirmed the formation of AgNPs in the reaction mixture. UV–Vis spectroscopy is one of the best applicable tools to confirm the formation of AgNPs. 198 3.1. UV-Vis Spectrum Moreover, surface plasmon resonance (SPR) patterns are frequently utilized as suggestive tools for metal 199 UV–Vis spectroscopy is one of the best applicable tools to confirm the formation of AgNPs. nanoparticles formation as SPR depends on a number of parameters, including size and medium dielectric 200 Moreover, surface plasmon resonance (SPR) patterns are frequently utilized as suggestive tools for constant [22]. The maximum absorption spectrum was determined between 300–800 nm (Figure 1). 201 metal nanoparticles formation as SPR depends on a number of parameters, including size and Moreover, characteristic peak at 400 nm in the UV-Vis spectrum was observed and this study specifically 202 medium dielectric constant [22]. The maximum absorption spectrum was determined between 300- pro2v0i3des8e0v0indmen(Fcieguorfef1o).rMmoarteiovnero, cfhbarioacstyernistthicepseizakedats4i0l0vnemr ninatnhoepUaVr-tVicislespsefcrtorumNwaigseolblasesravteidvansdeeds extract 204 this study specifically provides evidence of formation of biosynthesized silver nanoparticles from (Figure 1). This result is in consistent with the previous study, which exhibited that the synthesis of 205 Nigella sativa seeds extract (Figure 1). This result is in consistent with the previous study, which silver nanoparticles from Phoenix dactylifera (date palm) seeds and recorded the plasma resonance peak at 206 exhibited that the synthesis of silver nanoparticles from Phoenix dactylifera (date palm) seeds and 459 nm [11]. In this context, another study result revealed that a reduction of silver ions was observed 207 recorded the plasma resonance peak at 459 nm [11]. In this context, another study result revealed that bet2w08een 3a0r0edaunctdio6n0o0f snilmverainondspweaaskobasbersvoerdpbtieotwnewena3s00maondni6t0o0r nemd antd44p0eankmabsboyrpUtioVn-wVias smpoencitorroesdcopy [23]. 209 at 440 nm by UV-Vis spectroscopy [23]. 210 211 Figure 1. UV absorption spectrum of the AgNPs synthesized from Nigella sativa seed extract (Inset: Figure 1. UV absorption spectrum of the AgNPs synthesized from Nigella sativa seed extract (Inset: 212 Change of color from dark brownto light golden yellow). Change of color from dark brownto light golden yellow). 213 3.2. FTIR SpectroscopyPDF Image | Silver Nanoparticles Seed Extract Nigella sativa blackseed
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