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267 268 269 270 271 272 273 274 275 276 277 278 279 280 281 282 283 284 285 286 Processes 2020, 8, 388 8 of 14 Processes 2019, 7, x FOR PEER REVIEW 8 of 14 Figure 4. TEM images of Ns-AgNPs scale bar correspond to 0.5 μm (A) 100 nm (B) particle size Figure 4. TEM images of Ns-AgNPs scale bar correspond to 0.5 μm (A) 100 nm (B) particle size distribution by DLS (C). distribution by DLS (C). 3.5. Assessment of MIC and MBC Activity 3.5. Assessment of MIC and MBC activity Initially, the effect of Ns-AgNps was investigated against E. coli, K. pneumonia, P. aeruginosa, S. aureus Initially, the effect of Ns-AgNps was investigated against E. coli, K. pneumonia, P. aeruginosa, S. and E. faecalis bacterialstrains. Ns-AgNps showed the significant bacteriostatic and bactericidal activity. aureus and E. faecalis bacterialstrains. Ns-AgNps showed the significant bacteriostatic and bactericidal Even the lowest concentration of Ns-AgNps restricted the highest rate of inhibition against S. aureus activity. Even the lowest concentration of Ns-AgNps restricted the highest rate of inhibition against (6.5 and 15 μg/mL) and E. faecalis (6.5 and 15 μg/mL). Antimicrobial activity of S. aureus and E. fecalis S. aureus (6.5 and 15 μg/ml) and E. faecalis (6.5 and 15 μg/ml). Antimicrobial activity of S. aureus and was more pronounced than E. coli (15 and 30 μg/mL), K. pneumonia (15 and 30 μg/mL), and P. aeruginosa E. fecalis was more pronounced than E. coli (15 and 30 μg/ml), K. pneumonia (15 and 30 μg/ml), and (30 and 60 μg/mL) respectively (Table 1). In addition, Ns-AgNps concentration against above mentioned P. aeruginosa (30 and 60 μg/ml) respectively (Table 1). In addition, Ns-AgNps concentration against bacterial strains showed 6.5–30 μg/mL concentration for MIC and MBC values were 15–60 μg/mL. Our above mentioned bacterial strains showed 6.5–30 μg/ml concentration for MIC and MBC values were above-mentioned results revealed that Ns-AgNps exhibited excellent antibacterial activity and was 15-60 μg/ml. Our above-mentioned results revealed that Ns-AgNps exhibited excellent antibacterial in accordance with the findings of a previous study [35]. Silver nanoparticle play an important role activity and was in accordance with the findings of a previous study [35]. Silver nanoparticle play an in bactericidal, bacteriostatic and also kill the other types of pathogens. Sadeghi et al. evaluated the important role in bactericidal, bacteriostatic and also kill the other types of pathogens. Sadeghi et al. bactericidal effect of silver nanoparticles and chlorhexidine against S. mutans to observe that silver evaluated the bactericidal effect of silver nanoparticles and chlorhexidine against S. mutans to observe nanoparticles showed a statistically significant bactericidal effect as compared to chlorhexidine [36]. that silver nanoparticles showed a statistically significant bactericidal effect as compared to Table 1. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) activity of Ns-AgNps. Table 1. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) chlorhexidine [36]. activity of Ns-AgNps. S. No S. No Synthesized Ns-AgNps MIC (μg/mL) MBC (μg/mL) 1 2 3 43 54 5 Bacterial Strains Bacterial strains Klebsiella pneumonia (MTCC 618) Synthesized Ns-AgNps MIC (μg/ml) MBC(μg/ml) 1 2 Klebsiella pneumonia (MTCC 618) 15 30 Pseudomonas aeruginosa (MTCC 1688) 30 60 Pseudomonas aeruginosa (MTCC 1688) 30 60 Escherichia coli (MTCC 40) StaphEyslcochoecrciucshaiaurceoulsi(MTCC34106)0) StEanptheyrolococcoccucsufaseacaulriseu(Ms (TMCTCC4C393)160) 15 30 6.5 15 30 15 Enterococcus faecalis (MTCC 439) 6.5 6.5 15 6.5 15 15 15 30 3.6. Anti-Biofilm ActivityPDF Image | Silver Nanoparticles Seed Extract Nigella sativa blackseed
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