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287 288 289 290 291 292 293 294 295 296 297 3.6. Anti-Biofilm Activity Processes 2019, 7, x FOR PEER REVIEW 9 of 14 The results of tissue culture plate method demonstrated that antibiofilm activity of the biosynTtheesriezseudltAsgoNfPtisssfuroemcuNlt.urseatipvalatseemdeetxhtordactdewmaosn8s8tr.a4t2e%dftohratE.anfateibcaiolifsi,lm84.a9c2t%ivitfyoroEf.tchoeli, 8b1i.o86sy%ntfhoersKiz.epdnAeugmNoPnsiaf,roamndN8.2s.a8t4iv%a sfoeerdSe. xaturraecutsw, raess8p8e.c4t2i%veflyoraEt.1f2ae.5caμligs,/m84L.9,2w%hfioler Ew.ictohlit,h8e1.s8a6m%e cfoonrceKn.trpantieounm,otnhiae,baionfidlm82f.8o4r%maftoiornSw.asurreeuds,ucredspbeycti4v9e.l9y%aitn1t2h.e5cμagse/molf,Pw.aheirleugwiniothsat(hFeigusarme5e). Mconrecoenvetra,tihoen,evthideebnicoefiflomrsfocremeantiinogntwheasbiroefidlumceidnhbiybit4i9o.n9%initnhethperecsaesnecoefoPf.Naser-AuginNoPsasi(sFisghuorwen5)i.n Processes 2020, 8, 388 9 of 14 Moreover, the evidence for screening the biofilm inhibition in the presence of Ns-AgNPs is shown in Figure 6. These findings suggest that the uptake of the biosynthesised Ns-AgNPs could diminish the Figure 6. These findings suggest that the uptake of the biosynthesised Ns-AgNPs could diminish the biofilm forming abilities of tested pathogens. Similar studies were documented as results of biofilm biofilm forming abilities of tested pathogens. Similar studies were documented as results of biofilm inhibition at 100 μg/mL AgNPs [37]. Moreover, another study based on anti-biofilm demonstrated that inhibition at 100 μg/ml AgNPs [37]. Moreover, another study based on anti-biofilm demonstrated the AgNPs synthesized from Momordica charantia extract were exhibited improved anti-biofilm activity that the AgNPs synthesized from Momordica charantia extract were exhibited improved anti-biofilm against E.faecalis and A. hydrophilia. Additionally, the antibiofilm activity of AgNPs was attributed to activity against E.faecalis and A. hydrophilia. Additionally, the antibiofilm activity of AgNPs was the diffusion through the biofilms imparting antimicrobial actions [38]. attributed to the diffusion through the biofilms imparting antimicrobial actions [38]. K. pneumoniae E. fecalis 100 50 25 P. aeruginosa 12.5 S. aureus E.coli 0 20 40 60 80 100 120 Biofilm inhibition (%) 298 299 300 FFigiguurere55. .Anttii--biiofifilm activity by using difffferent concentrations (12..5,, 25,, 50,, 100 μg/mlL))ooffssilivlveerr nnaannooppaarrtitciclelessssyynntthheessiizzeedffrromBllackSeedextract against difffferent standard bacterial strains. Ag-NPs (μg/ml)PDF Image | Silver Nanoparticles Seed Extract Nigella sativa blackseed
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